Bacterial Expression of Mouse Argonaute 2 for Functional and Mutational Studies
Abstract
:1. Introduction
2. Results and Discussion
2.1. Expression in E. Coli and Purification of Mouse Argonaute 2
2.2. Functional Characterization of Mouse Argonaute 2
2.3. Mutational Analysis of mAgo2
3. Experimental Section
3.1. Materials and General Procedures
3.2. DNA Cloning and Site-Directed Mutagenesis
3.3. Protein Expression and Purification
3.4. Structural and Functional Analyses
4. Conclusions
Acknowledgments
References and Notes
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Salvatore, V.; Potenza, N.; Papa, U.; Nobile, V.; Russo, A. Bacterial Expression of Mouse Argonaute 2 for Functional and Mutational Studies. Int. J. Mol. Sci. 2010, 11, 745-753. https://doi.org/10.3390/ijms11020745
Salvatore V, Potenza N, Papa U, Nobile V, Russo A. Bacterial Expression of Mouse Argonaute 2 for Functional and Mutational Studies. International Journal of Molecular Sciences. 2010; 11(2):745-753. https://doi.org/10.3390/ijms11020745
Chicago/Turabian StyleSalvatore, Vincenzo, Nicoletta Potenza, Umberto Papa, Valentina Nobile, and Aniello Russo. 2010. "Bacterial Expression of Mouse Argonaute 2 for Functional and Mutational Studies" International Journal of Molecular Sciences 11, no. 2: 745-753. https://doi.org/10.3390/ijms11020745