Abstract: Previous studies have determined that lin-4, which was the first miRNA to be discovered, controls the timing of cell fate determination and life span in Caenorhabditis elegans. However, the mechanism of lin-4 involvement in these processes remains poorly understood. Fat storage is an essential aspect of the life cycle of organisms, and the function of lin-4 in fat accumulation is not clear. In this study, we showed that the fat content is reduced remarkably in C. elegans lin-4 mutants. Quantitative RT-PCR analysis revealed a considerable decrease in the levels of SBP-1 and OGA-1 mRNA in lin-4 mutants. We also showed that lin-4 mutants have a significantly shorter life span than wild-type worms. DCF assay experiments showed that the reactive oxygen species (ROS) levels increased and mitochondrial DNA (mtDNA) copy number decreased in loss-of-function lin-4 mutants. These mutants also showed attenuation of locomotion. Taken together, our findings suggest that lin-4 may play an important role in regulating fat accumulation and locomotion and that lin-4 may control the life span of C. elegans by mediating ROS production.
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Zhu, C.; Ji, C.-B.; Zhang, C.-M.; Gao, C.-L.; Zhu, J.-G.; Qin, D.-N.; Kou, C.-Z.; Zhu, G.-Z.; Shi, C.-M.; Guo, X.-R. The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans . Int. J. Mol. Sci. 2010, 11, 4814-4825.
Zhu C, Ji C-B, Zhang C-M, Gao C-L, Zhu J-G, Qin D-N, Kou C-Z, Zhu G-Z, Shi C-M, Guo X-R. The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans . International Journal of Molecular Sciences. 2010; 11(12):4814-4825.
Zhu, Chun; Ji, Chen-Bo; Zhang, Chun-Mei; Gao, Chun-Lin; Zhu, Jin-Gai; Qin, Da-Ni; Kou, Chun-Zhao; Zhu, Guan-Zhong; Shi, Chun-Mei; Guo, Xi-Rong. 2010. "The lin-4 Gene Controls Fat Accumulation and Longevity in Caenorhabditis elegans ." Int. J. Mol. Sci. 11, no. 12: 4814-4825.