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• Cotar, A
• Chifiriuc, M
• Dinu, S
• Bucur, M
• Iordache, C
• Banu, O
• Dracea, O
• Larion, C
• Lazar, V
• [+] on Google Scholar
• Cotar, A
• Chifiriuc, M
• Dinu, S
• Bucur, M
• Iordache, C
• Banu, O
• Dracea, O
• Larion, C
• Lazar, V
• [+] on PubMed
• Cotar, A
• Chifiriuc, M
• Dinu, S
• Bucur, M
• Iordache, C
• Banu, O
• Dracea, O
• Larion, C
• Lazar, V

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Int. J. Mol. Sci. 2010, 11(12), 5273-5291; doi:10.3390/ijms11125273

Article

Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections

Ani-Ioana Cotar ¹ , Mariana-Carmen Chifiriuc ^2,* , Sorin Dinu ¹ , Marcela Bucur ² , Carmen Iordache ¹ , Otilia Banu ³ , Olguta Dracea ¹ , Cristina Larion ¹  and Veronica Lazar ²

¹ National Institute for Research in Microbiology and Immunology, Cantacuzino, Spl. Independentei 103, cod 060631, Bucharest 060101, Romania ² Department of Microbiology, Faculty of Biology, University of Bucharest, Aleea Portocalelor 1-3, Bucharest 060101, Romania ³ Institute for Cardiovascular Diseases Prof. C.C. Iliescu, Bucharest 060101, Romania

* Author to whom correspondence should be addressed.

Received: 12 November 2010; in revised form: 2 December 2010 / Accepted: 3 December 2010 / Published: 21 December 2010

(This article belongs to the Special Issue Advances in Molecular Diagnostics)

Download PDF Full-Text [538 KB, uploaded 21 December 2010 15:39 CET]

Abstract: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.

Keywords: Staphylococcus aureus; Pseudomonas aeruginosa; virulence; molecular markers; invasive infections

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