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Int. J. Mol. Sci. 2010, 11(12), 5273-5291; doi:10.3390/ijms11125273
Article

Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections

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Received: 12 November 2010; in revised form: 2 December 2010 / Accepted: 3 December 2010 / Published: 21 December 2010
(This article belongs to the Special Issue Advances in Molecular Diagnostics)
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Abstract: Staphylococcus (S.) aureus and Pseudomonas (Ps.) aeruginosa are two of the most frequently opportunistic pathogens isolated in nosocomial infections, responsible for severe infections in immunocompromised hosts. The frequent emergence of antibiotic-resistant S. aureus and Ps. aeruginosa strains has determined the development of new strategies in order to elucidate the different mechanisms used by these bacteria at different stages of the infectious process, providing the scientists with new procedures for preventing, or at least improving, the control of S. aureus and Ps. aeruginosa infections. The purpose of this study was to characterize the molecular markers of virulence in S. aureus and Ps. aeruginosa strains isolated from different clinical specimens. We used multiplex and uniplex PCR assays to detect the genes encoding different cell-wall associated and extracellular virulence factors, in order to evaluate potential associations between the presence of putative virulence genes and the outcome of infections caused by these bacteria. Our results demonstrate that all the studied S. aureus and Ps. aeruginosa strains synthesize the majority of the investigated virulence determinants, probably responsible for different types of infections.
Keywords: Staphylococcus aureus; Pseudomonas aeruginosa; virulence; molecular markers; invasive infections Staphylococcus aureus; Pseudomonas aeruginosa; virulence; molecular markers; invasive infections
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Cotar, A.-I.; Chifiriuc, M.-C.; Dinu, S.; Bucur, M.; Iordache, C.; Banu, O.; Dracea, O.; Larion, C.; Lazar, V. Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections. Int. J. Mol. Sci. 2010, 11, 5273-5291.

AMA Style

Cotar A-I, Chifiriuc M-C, Dinu S, Bucur M, Iordache C, Banu O, Dracea O, Larion C, Lazar V. Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections. International Journal of Molecular Sciences. 2010; 11(12):5273-5291.

Chicago/Turabian Style

Cotar, Ani-Ioana; Chifiriuc, Mariana-Carmen; Dinu, Sorin; Bucur, Marcela; Iordache, Carmen; Banu, Otilia; Dracea, Olguta; Larion, Cristina; Lazar, Veronica. 2010. "Screening of Molecular Virulence Markers in Staphylococcus aureus and Pseudomonas aeruginosa Strains Isolated from Clinical Infections." Int. J. Mol. Sci. 11, no. 12: 5273-5291.



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