Int. J. Mol. Sci. 2010, 11(11), 4741-4749; doi:10.3390/ijms11114741
Brief Report

Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats

1,* email, 1email and 2email
Received: 20 October 2010; in revised form: 16 November 2010 / Accepted: 17 November 2010 / Published: 19 November 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Copper (Cu) transporter 2 (Ctr2) is a transmembrane protein that transports Cu across cell membranes and increases cytosolic Cu levels. Experiments using cell lines have suggested that Ctr2 expression is regulated by Cu status. The importance of changes in Ctr2 expression is underscored by recent studies demonstrating that lower Ctr2 content in cells increases the cellular uptake of platinum-containing cancer drugs and toxicity to the drugs. In this study, we examined whether Ctr2 expression is altered by a nutritional Cu deficiency in vivo. Ctr2 mRNA and protein in liver and heart from rats fed a normal (Cu-N), moderately deficient (Cu-M) or deficient (Cu-D) Cu diet was measured. Rats fed the Cu-deficient diets showed a dose-dependent decrease in liver Ctr2 protein compared to Cu-N rats. Ctr2 protein was 42% and 85% lower in Cu-M and Cu-D rats, respectively. Liver Ctr2 mRNA was 50% lower in Cu-D rats and unaffected in Cu-M rats. In heart, Ctr2 protein was only lower in Cu-D rats (46% lower). These data show that Cu deficiency decreases Ctr2 content in vivo.
Keywords: Ctr2; copper; rat; diet; expression
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MDPI and ACS Style

Bertinato, J.; Duval, S.; L’Abbé, M.R. Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats. Int. J. Mol. Sci. 2010, 11, 4741-4749.

AMA Style

Bertinato J, Duval S, L’Abbé MR. Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats. International Journal of Molecular Sciences. 2010; 11(11):4741-4749.

Chicago/Turabian Style

Bertinato, Jesse; Duval, Sébastien; L’Abbé, Mary R. 2010. "Copper Transporter 2 Content Is Lower in Liver and Heart of Copper-Deficient Rats." Int. J. Mol. Sci. 11, no. 11: 4741-4749.


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