Int. J. Mol. Sci. 2010, 11(11), 4750-4763; doi:10.3390/ijms11114750
Article

Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis

1email, 2email, 3email, 1email, 1email and 1,2,* email
Received: 21 September 2010; in revised form: 26 October 2010 / Accepted: 15 November 2010 / Published: 22 November 2010
(This article belongs to the Section Bioactives and Nutraceuticals)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Optimization of total flavonoid compound (TFC) extraction from Gynura medica leaf was investigated using response surface methodology (RSM) in this paper. The conditions investigated were 30–60% (v/v) ethanol concentration (X1), 8595 °C extraction temperature (X2) and 30–50 (v/w) liquid-to-solid ratio (X3). Statistical analysis of the experiments indicated that temperature and liquid-to-solid ratio significantly affected TFC extraction (p < 0.01). The Box-Behnken experiment design showed that polynomial regression models were in good agreement with the experimental results, with the coefficients of determination of 0.9325 for TFC yield. The optimal conditions for maximum TFC yield were 55% ethanol, 92 °C and 50 (v/w) liquid-to-solid ratio with a 30 min extraction time. Extracts from these conditions showed a moderate antioxidant value of 54.78 μmol quercetin/g dry material (DM), 137.3 μmol trolox/g DM for 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 108.21 μmol quercetin/g DM, 242.31 μmol trolox/g DM for 2,2-azino-bis-(3-ethylbenzthiazoline-6-sulphonic acid) (ABTS+), respectively. HPLC-DAD-MS analysis showed that kaempferol-3-O-glucoside was the principal flavonoid compound in Gynura medica leaf.
Keywords: Gynura medica; response surface methodology; flavonoid compounds; antioxidant activity
PDF Full-text Download PDF Full-Text [534 KB, uploaded 19 June 2014 03:19 CEST]

Export to BibTeX |
EndNote


MDPI and ACS Style

Liu, W.; Yu, Y.; Yang, R.; Wan, C.; Xu, B.; Cao, S. Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis. Int. J. Mol. Sci. 2010, 11, 4750-4763.

AMA Style

Liu W, Yu Y, Yang R, Wan C, Xu B, Cao S. Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis. International Journal of Molecular Sciences. 2010; 11(11):4750-4763.

Chicago/Turabian Style

Liu, Wei; Yu, Yanying; Yang, Ruzhen; Wan, Chunpeng; Xu, Binbin; Cao, Shuwen. 2010. "Optimization of Total Flavonoid Compound Extraction from Gynura medica Leaf Using Response Surface Methodology and Chemical Composition Analysis." Int. J. Mol. Sci. 11, no. 11: 4750-4763.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert