Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection
Abstract
:1. Introduction
2. Results and Discussions
2.1. Effect of Microwave Assisted Acid Pre-Treatment on Bone Collagen
2.2. Effect of Microwave Assisted Acid Pre-Treatment on Enzyme Digestion of Bone Collagen
2.3. Effect of Microwave Assisted Acid Pre-treatment on the Antioxidant Activity
2.4. Effect of Nitrogen Protection on DH and Antioxidation Activity
3. Experimental
3.1. Materials
3.2. Preparation of Collagen Peptides
3.2.1. Microwave Assisted Acid Pre-treatment of Bone Collagen
3.2.2. Protease Hydrolysis of Pre-Treatment of Bone Collagen
3.3. Determination of Molecular Weight Distribution (MW)
3.4. DPPH Radicals Scavenging Activity Assay
3.5. ABTS• Scavenging Activity Assay
3.6. Statistical Analysis
4. Conclusion
Acknowledgments
References
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Hydrochloric Acid | Sulfuric Acid | Phosphoric Acid | Citric Acid | |
---|---|---|---|---|
DH (%) | 5.22 ± 0.18 | 4.80 ± 0.15 | 6.17 ± 0.08 | 4.16 ± 0.21 |
ABTS+ (%) | 6.5 ± 0.23 | 4.8 ± 1.02 | 28.0 ± 0.11 | 21.2 ± 0.12 |
DPPH·(%) | 9.2 ± 0.17 | 12.1 ± 0.09 | 9.9 ± 0.09 | 10.1 ± 0.10 |
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Lin, Y.-J.; Le, G.-W.; Wang, J.-Y.; Li, Y.-X.; Shi, Y.-H.; Sun, J. Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection. Int. J. Mol. Sci. 2010, 11, 4297-4308. https://doi.org/10.3390/ijms11114297
Lin Y-J, Le G-W, Wang J-Y, Li Y-X, Shi Y-H, Sun J. Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection. International Journal of Molecular Sciences. 2010; 11(11):4297-4308. https://doi.org/10.3390/ijms11114297
Chicago/Turabian StyleLin, Yun-Jian, Guo-Wei Le, Jie-Yun Wang, Ya-Xin Li, Yong-Hui Shi, and Jin Sun. 2010. "Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection" International Journal of Molecular Sciences 11, no. 11: 4297-4308. https://doi.org/10.3390/ijms11114297