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Int. J. Mol. Sci. 2010, 11(11), 4297-4308; doi:10.3390/ijms11114297
Article

Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection

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Received: 18 August 2010; in revised form: 21 October 2010 / Accepted: 21 October 2010 / Published: 1 November 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract: This study focused on the preparation method of antioxidant peptides by enzymatic hydrolysis of bone collagen after microwave assisted acid pre-treatment and nitrogen protection. Phosphoric acid showed the highest ability of hydrolysis among the four other acids tested (hydrochloric acid, sulfuric acid and/or citric acid). The highest degree of hydrolysis (DH) was 9.5% using 4 mol/L phosphoric acid with a ratio of 1:6 under a microwave intensity of 510 W for 240 s. Neutral proteinase gave higher DH among the four protease tested (Acid protease, neutral protease, Alcalase and papain), with an optimum condition of: (1) ratio of enzyme and substrate, 4760 U/g; (2) concentration of substrate, 4%; (3) reaction temperature, 55 °C and (4) pH 7.0. At 4 h, DH increased significantly (P < 0.01) under nitrogen protection compared with normal microwave assisted acid pre-treatment hydrolysis conditions. The antioxidant ability of the hydrolysate increased and reached its maximum value at 3 h; however DH decreased dramatically after 3 h. Microwave assisted acid pre-treatment and nitrogen protection could be a quick preparatory method for hydrolyzing bone collagen.
Keywords: bone collagen; nitrogen protection; microwave assisted acid pre-treatment; enzyme hydrolysis; antioxidant activity bone collagen; nitrogen protection; microwave assisted acid pre-treatment; enzyme hydrolysis; antioxidant activity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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MDPI and ACS Style

Lin, Y.-J.; Le, G.-W.; Wang, J.-Y.; Li, Y.-X.; Shi, Y.-H.; Sun, J. Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection. Int. J. Mol. Sci. 2010, 11, 4297-4308.

AMA Style

Lin Y-J, Le G-W, Wang J-Y, Li Y-X, Shi Y-H, Sun J. Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection. International Journal of Molecular Sciences. 2010; 11(11):4297-4308.

Chicago/Turabian Style

Lin, Yun-Jian; Le, Guo-Wei; Wang, Jie-Yun; Li, Ya-Xin; Shi, Yong-Hui; Sun, Jin. 2010. "Antioxidative Peptides Derived from Enzyme Hydrolysis of Bone Collagen after Microwave Assisted Acid Pre-Treatment and Nitrogen Protection." Int. J. Mol. Sci. 11, no. 11: 4297-4308.



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