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Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis
Liaoning Ocean and Fisheries Science Research Institute, The Key Laboratory of Marine Fishery Molecular Biology of Liaoning province, Dalian 116023, China
Institute of Chemical and Physics, Chinese Academy of Sciences, Dalian 116023, China
College of Agriculture, Auburn University, Auburn, AL 36849, USA
* Author to whom correspondence should be addressed.
Received: 19 September 2010; in revised form: 1 October 2010 / Accepted: 1 October 2010 / Published: 15 October 2010
Abstract: Japanese scallop (Mizuhopecten yessoensis) is a cold-tolerant bivalve that was introduced to China for aquaculture in 1982. In this study, amplified fragment length polymorphism (AFLP) markers were used to investigate levels of genetic diversity within M. yessoensis cultured stocks and compare them with wild populations. Six pairs of primer combinations generated 368 loci among 332 individuals, in four cultured and three wild populations. High polymorphism at AFLP markers was found within both cultured and wild M. yessoensis populations. The percentage of polymorphic loci ranged from 61.04% to 72.08%, while the mean heterozygosity ranged from 0.2116 to 0.2596. Compared with wild populations, the four hatchery populations showed significant genetic changes, such as lower expected heterozygosity and percentage of polymorphic loci, and smaller frequency of private alleles, all indicative of a reduction in genetic diversity. Some genetic structures were associated with the geographical distribution of samples; with all samples from Dalian and Japan being closely related, while the population from Russia fell into a distinct clade in the phylogenetic analysis. The genetic information derived from this study indicated that intentional or accidental release of selected Japanese scallops into natural sea areas might result in disturbance of local gene pools and loss of genetic variability. We recommend monitoring the genetic variability of selected hatchery populations to enhance conservation of natural Japanese scallop resources.
Keywords: Japanese scallop; Mizuhopecten yessoensis; AFLP; genetic diversity; population structure; selective breeding
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MDPI and ACS Style
Liu, W.-D.; Li, H.-J.; Bao, X.-B.; Gao, X.-G.; Li, Y.-F.; He, C.-B.; Liu, Z.-J. Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis. Int. J. Mol. Sci. 2010, 11, 3933-3941.
Liu W-D, Li H-J, Bao X-B, Gao X-G, Li Y-F, He C-B, Liu Z-J. Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis. International Journal of Molecular Sciences. 2010; 11(10):3933-3941.
Liu, Wei-Dong; Li, Hong-Jun; Bao, Xiang-Bo; Gao, Xiang-gang; Li, Yun-feng; He, Chong-Bo; Liu, Zhan-Jiang. 2010. "Genetic Differentiation between Natural and Hatchery Stocks of Japanese Scallop (Mizuhopecten yessoensis) as Revealed by AFLP Analysis." Int. J. Mol. Sci. 11, no. 10: 3933-3941.