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Int. J. Mol. Sci. 2010, 11(10), 3922-3932; doi:10.3390/ijms11103922

Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Pseudomonas aeruginosa Using the Evolutionary Operation-Factorial Design Technique

1
Pohang Center for Evaluation of Biomaterials, Pohang 790-834, Korea
2
Department of Food Science and Biotechnology, Kangwon national University, Chuncheon, 200-701, Korea
3
Korea Food Research Institute, Seongnam, Kyonggi, 463-746, Korea
*
Authors to whom correspondence should be addressed.
Received: 7 September 2010 / Revised: 20 September 2010 / Accepted: 13 October 2010 / Published: 14 October 2010
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

This study was undertaken to optimize the conditions for the extraction of antibacterial activity of Perilla frutescens var. acuta leaf against Pseudomonas aeruginosa KCTC 2004 using the evolutionary operation-factorial (EVOP) design technique. Increased antibacterial activity was achieved at higher extraction temperatures and with a longer extraction time. Antibacterial activity was not affected by differing ethanol concentration in the extraction solvent. The maximum antibacterial activity of ethanolic extract of P. frutescens var. acuta leaf against P. aeruginosa, determined by the EVOP factorial technique, was obtained at an extraction temperature of 80 °C (R = −0.800**), 26 h (R = −0.731**) extraction time, and 50% (R = −0.075) ethanol concentration. The population of P. aeruginosa also decreased from 6.660 log CFU/mL in the initial set to 4.060 log CFU/mL in the third set. Also, scanning electron microscopy study of the ethanolic extract of P. frutescens var. acuta revealed potential detrimental effects on the morphology of P. aeruginosa.
Keywords: Perilla frutescens var. acuta leaf; EVOP-factorial design technique; antibacterial activity; Pseudomonas aeruginosa KCTC 2004 Perilla frutescens var. acuta leaf; EVOP-factorial design technique; antibacterial activity; Pseudomonas aeruginosa KCTC 2004
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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MDPI and ACS Style

Choi, U.-K.; Lee, O.-H.; Lim, S.-I.; Kim, Y.-C. Optimization of Antibacterial Activity of Perilla frutescens var. acuta Leaf against Pseudomonas aeruginosa Using the Evolutionary Operation-Factorial Design Technique. Int. J. Mol. Sci. 2010, 11, 3922-3932.

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