Int. J. Mol. Sci. 2009, 10(8), 3269-3282; doi:10.3390/ijms10083269
Article

Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms

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Received: 5 June 2009; in revised form: 7 July 2009 / Accepted: 17 July 2009 / Published: 24 July 2009
(This article belongs to the Special Issue Biotic and Abiotic Stress)
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Phytochelatins (PCs) play an important role in detoxification of heavy metals in plants. PCs are synthesized from glutathione by phytochelatin synthase (PCS), a dipeptidyltransferase. Sesbania rostrata is a tropical legume plant that can tolerate high concentrations of Cd and Zn. In this study, the S. rostrata PCS gene (SrPCS) and cDNAs were isolated and characterized. Southern blot and sequence analysis revealed that a single copy of the SrPCS gene occurs in the S. rostrata genome, and produces four different SrPCS mRNAs and proteins, SrPCS1-SrPCS4, by alternative splicing of the SrPCS pre-mRNA. The SrPCS1 and SrPCS3 proteins conferred Cd tolerance when expressed in yeast cells, whereas the SrPCS2 and SrPCS4 proteins, which lack the catalytic triad and the N-terminal domains, did not. These results suggested that SrPCS1 and SrPCS3 have potential applications in genetic engineering of plants for enhancing heavy metal tolerance and phytoremediation of contaminated soils.
Keywords: alternative splicing; heavy metal tolerance; phytochelatin; phytochelatin synthase; phytoremediation; Sesbania rostrata
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MDPI and ACS Style

Li, A.-M.; Yu, B.-Y.; Chen, F.-H.; Gan, H.-Y.; Yuan, J.-G.; Qiu, R.; Huang, J.-C.; Yang, Z.-Y.; Xu, Z.-F. Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms. Int. J. Mol. Sci. 2009, 10, 3269-3282.

AMA Style

Li A-M, Yu B-Y, Chen F-H, Gan H-Y, Yuan J-G, Qiu R, Huang J-C, Yang Z-Y, Xu Z-F. Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms. International Journal of Molecular Sciences. 2009; 10(8):3269-3282.

Chicago/Turabian Style

Li, An-Ming; Yu, Bing-Yun; Chen, Fu-Hua; Gan, Hui-Yan; Yuan, Jian-Gang; Qiu, Rongliang; Huang, Jun-Chao; Yang, Zhong-Yi; Xu, Zeng-Fu. 2009. "Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms." Int. J. Mol. Sci. 10, no. 8: 3269-3282.

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