Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms
Abstract
:1. Introduction
2. Results and Discussion
2.1. Cloning and Sequence Analysis of PCS cDNAs and Gene from S. rostrata
2.2. Genomic Organization and Pre-mRNA Splicing of SrPCS Gene
2.3. Expression Analysis of SrPCS Gene
2.4. Functionality of Four SrPCS Isoforms SrPCS1–SrPCS4
3. Experimental Section
3.1. Plant Materials
3.2. Cloning of PCS cDNAs and Gene from S. rostrata
3.3. Real Time RT-PCR of SrPCS3
3.4. Southern Blot Analysis of SrPCS
3.5. Expression of Recombinant SrPCS1-4 in Yeast and Cd Tolerance Assay
4. Conclusions
Acknowledgments
References
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Li, A.-M.; Yu, B.-Y.; Chen, F.-H.; Gan, H.-Y.; Yuan, J.-G.; Qiu, R.; Huang, J.-C.; Yang, Z.-Y.; Xu, Z.-F. Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms. Int. J. Mol. Sci. 2009, 10, 3269-3282. https://doi.org/10.3390/ijms10083269
Li A-M, Yu B-Y, Chen F-H, Gan H-Y, Yuan J-G, Qiu R, Huang J-C, Yang Z-Y, Xu Z-F. Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms. International Journal of Molecular Sciences. 2009; 10(8):3269-3282. https://doi.org/10.3390/ijms10083269
Chicago/Turabian StyleLi, An-Ming, Bing-Yun Yu, Fu-Hua Chen, Hui-Yan Gan, Jian-Gang Yuan, Rongliang Qiu, Jun-Chao Huang, Zhong-Yi Yang, and Zeng-Fu Xu. 2009. "Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms" International Journal of Molecular Sciences 10, no. 8: 3269-3282. https://doi.org/10.3390/ijms10083269