Next Article in Journal
Classification of 5-HT1A Receptor Ligands on the Basis of Their Binding Affinities by Using PSO-Adaboost-SVM
Next Article in Special Issue
Analysis of Cooperativity by Isothermal Titration Calorimetry
Previous Article in Journal
Characterization of the Sesbania rostrata Phytochelatin Synthase Gene: Alternative Splicing and Function of Four Isoforms
Previous Article in Special Issue
Applications of Isothermal Titration Calorimetry in Biophysical Studies of G-quadruplexes
Int. J. Mol. Sci. 2009, 10(8), 3283-3315; doi:10.3390/ijms10083283

Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry

1,2,*  and 3
1 Institut National de la Santé et de la Recherche Médicale, Unité mixte de recherche 977, 11 rue Humann, 67085 Strasbourg Cédex, France 2 Université de Strasbourg, Faculté de Chirurgie Dentaire, 1 Place de l’Hôpital, 67000 Strasbourg, France 3 Laboratoire d’Innovation Thérapeutique, Unité Mixte de Recherche 7200 CNRS - Université de Strasbourg, Faculté de Pharmacie, 74 route du Rhin BP 60024, F-67401 ILLKIRCH Cedex, France
* Author to whom correspondence should be addressed.
Received: 3 June 2009 / Revised: 21 July 2009 / Accepted: 24 July 2009 / Published: 28 July 2009
(This article belongs to the Special Issue Isothermal Titration Calorimetry)
View Full-Text   |   Download PDF [340 KB, uploaded 19 June 2014]   |  


Isothermal titration microcalorimetry (ITC) is mostly used to investigate the thermodynamics of “specific” host-guest interactions in biology as well as in supramolecular chemistry. The aim of this review is to demonstrate that ITC can also provide useful information about non-specific interactions, like electrostatic or hydrophobic interactions. More attention will be given in the use of ITC to investigate polyelectrolyte-polyelectrolyte (in particular DNA-polycation), polyelectrolyte-protein as well as protein-lipid interactions. We will emphasize that in most cases these “non specific” interactions, as their definition will indicate, are favoured or even driven by an increase in the entropy of the system. The origin of this entropy increase will be discussed for some particular systems. We will also show that in many cases entropy-enthalpy compensation phenomena occur.
Keywords: non-specific interactions; isothermal titration calorimetry non-specific interactions; isothermal titration calorimetry
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

Share & Cite This Article

Further Mendeley | CiteULike
Export to BibTeX |
EndNote |
MDPI and ACS Style

Ball, V.; Maechling, C. Isothermal Microcalorimetry to Investigate Non Specific Interactions in Biophysical Chemistry. Int. J. Mol. Sci. 2009, 10, 3283-3315.

View more citation formats

Related Articles

Article Metrics

For more information on the journal, click here


[Return to top]
Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert