Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays
AbstractQuantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.
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Giraud, G.; Schulze, H.; Bachmann, T.T.; Campbell, C.J.; Mount, A.R.; Ghazal, P.; Khondoker, M.R.; Ross, A.J.; Ember, S.W.J.; Ciani, I.; Tlili, C.; Walton, A.J.; Terry, J.G.; Crain, J. Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays. Int. J. Mol. Sci. 2009, 10, 1930-1941.
Giraud G, Schulze H, Bachmann TT, Campbell CJ, Mount AR, Ghazal P, Khondoker MR, Ross AJ, Ember SWJ, Ciani I, Tlili C, Walton AJ, Terry JG, Crain J. Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays. International Journal of Molecular Sciences. 2009; 10(4):1930-1941.Chicago/Turabian Style
Giraud, Gerard; Schulze, Holger; Bachmann, Till T.; Campbell, Colin J.; Mount, Andrew R.; Ghazal, Peter; Khondoker, Mizanur R.; Ross, Alan J.; Ember, Stuart W.J.; Ciani, Ilenia; Tlili, Chaker; Walton, Anthony J.; Terry, Jonathan G.; Crain, Jason. 2009. "Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays." Int. J. Mol. Sci. 10, no. 4: 1930-1941.