Int. J. Mol. Sci. 2009, 10(4), 1930-1941; doi:10.3390/ijms10041930

Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays

1,* email, 2email, 2email, 2email, 3email, 2email, 2email, 2email, 2email, 3email, 3email, 4email, 4email and 1, 5email
Received: 4 March 2009; in revised form: 16 April 2009 / Accepted: 21 April 2009 / Published: 24 April 2009
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
Abstract: Quantum dot (QD) labeling combined with fluorescence lifetime imaging microscopy is proposed as a powerful transduction technique for the detection of DNA hybridization events. Fluorescence lifetime analysis of DNA microarray spots of hybridized QD labeled target indicated a characteristic lifetime value of 18.8 ns, compared to 13.3 ns obtained for spots of free QD solution, revealing that QD labels are sensitive to the spot microenvironment. Additionally, time gated detection was shown to improve the microarray image contrast ratio by 1.8, achieving femtomolar target sensitivity. Finally, lifetime multiplexing based on Qdot525 and Alexa430 was demonstrated using a single excitation-detection readout channel.
Keywords: Quantum Dots (QD); Fluorescence Lifetime Imaging Microscopy (FLIM); DNA Microarray; Lifetime Multiplexing
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MDPI and ACS Style

Giraud, G.; Schulze, H.; Bachmann, T.T.; Campbell, C.J.; Mount, A.R.; Ghazal, P.; Khondoker, M.R.; Ross, A.J.; Ember, S.W.J.; Ciani, I.; Tlili, C.; Walton, A.J.; Terry, J.G.; Crain, J. Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays. Int. J. Mol. Sci. 2009, 10, 1930-1941.

AMA Style

Giraud G, Schulze H, Bachmann TT, Campbell CJ, Mount AR, Ghazal P, Khondoker MR, Ross AJ, Ember SWJ, Ciani I, Tlili C, Walton AJ, Terry JG, Crain J. Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays. International Journal of Molecular Sciences. 2009; 10(4):1930-1941.

Chicago/Turabian Style

Giraud, Gerard; Schulze, Holger; Bachmann, Till T.; Campbell, Colin J.; Mount, Andrew R.; Ghazal, Peter; Khondoker, Mizanur R.; Ross, Alan J.; Ember, Stuart W.J.; Ciani, Ilenia; Tlili, Chaker; Walton, Anthony J.; Terry, Jonathan G.; Crain, Jason. 2009. "Fluorescence Lifetime Imaging of Quantum Dot Labeled DNA Microarrays." Int. J. Mol. Sci. 10, no. 4: 1930-1941.

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