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Int. J. Mol. Sci. 2009, 10(2), 395-406; doi:10.3390/ijms10020395
Article

Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes

1,2, 2
, 2
, 3
 and 1,*
1 Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México 2 Laboratorio de Farmacología, Instituto de Ciencias de la Salud, UAEH., Ex-Hacienda de la Concepción. Tilcuautla. Pachuca de Soto, Hgo. Cp 42080, México 3 Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México
* Author to whom correspondence should be addressed.
Received: 21 November 2008 / Revised: 5 January 2009 / Accepted: 6 January 2009 / Published: 1 February 2009
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Abstract

Aflatoxin B1 is a contaminant of agricultural and dairy products that can be related to mutagenic and carcinogenic effects. In this report we explore the capacity of α-mannan (Man) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose we applied the comet assay to groups of animals which were first administered Man (100, 400 and 700 mg/kg, respectively) and 20 min later 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10, and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The obtained data suggested the formation of a supramolecular complex between AFB1 and Man.
Keywords: Aflatoxin B1; mannan; mouse hepatocytes; antigenotoxicity Aflatoxin B1; mannan; mouse hepatocytes; antigenotoxicity
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Madrigal-Santillán, E.; Morales-González, J.A.; Sánchez-Gutiérrez, M.; Reyes-Arellano, A.; Madrigal-Bujaidar, E. Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes. Int. J. Mol. Sci. 2009, 10, 395-406.

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