Int. J. Mol. Sci. 2009, 10(2), 395-406; doi:10.3390/ijms10020395
Article

Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes

Eduardo Madrigal-Santillán 1,2 , José Antonio Morales-González 2 email, Manuel Sánchez-Gutiérrez 2 email, Alicia Reyes-Arellano 3 email and Eduardo Madrigal-Bujaidar 1,* email
1 Laboratorio de Genética, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México 2 Laboratorio de Farmacología, Instituto de Ciencias de la Salud, UAEH., Ex-Hacienda de la Concepción. Tilcuautla. Pachuca de Soto, Hgo. Cp 42080, México 3 Departamento de Química Orgánica, Escuela Nacional de Ciencias Biológicas, I.P.N., Carpio y Plan de Ayala. Sto. Tomas, México D.F. Cp 11340, México
* Author to whom correspondence should be addressed.
Received: 21 November 2008; in revised form: 5 January 2009 / Accepted: 6 January 2009 / Published: 1 February 2009
(This article belongs to the Special Issue Mycotoxins: Mechanisms of Toxicological Activity - Treatment and Prevention)
PDF Full-text Download PDF Full-Text [363 KB, uploaded 1 February 2009 17:13 CET]
Abstract: Aflatoxin B1 is a contaminant of agricultural and dairy products that can be related to mutagenic and carcinogenic effects. In this report we explore the capacity of α-mannan (Man) to reduce the DNA damage induced by AFB1 in mouse hepatocytes. For this purpose we applied the comet assay to groups of animals which were first administered Man (100, 400 and 700 mg/kg, respectively) and 20 min later 1.0 mg/kg of AFB1. Liver cells were obtained at 4, 10, and 16 h after the chemical administration and examined. The results showed no protection of the damage induced by AFB1 with the low dose of the polysaccharide, but they did reveal antigenotoxic activity exerted by the two high doses. In addition, we induced a co-crystallization between both compounds, determined their fusion points and analyzed the molecules by UV spectroscopy. The obtained data suggested the formation of a supramolecular complex between AFB1 and Man.
Keywords: Aflatoxin B1; mannan; mouse hepatocytes; antigenotoxicity

Article Statistics

Click here to load and display the download statistics.

Cite This Article

MDPI and ACS Style

Madrigal-Santillán, E.; Morales-González, J.A.; Sánchez-Gutiérrez, M.; Reyes-Arellano, A.; Madrigal-Bujaidar, E. Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes. Int. J. Mol. Sci. 2009, 10, 395-406.

AMA Style

Madrigal-Santillán E, Morales-González JA, Sánchez-Gutiérrez M, Reyes-Arellano A, Madrigal-Bujaidar E. Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes. International Journal of Molecular Sciences. 2009; 10(2):395-406.

Chicago/Turabian Style

Madrigal-Santillán, Eduardo; Morales-González, José Antonio; Sánchez-Gutiérrez, Manuel; Reyes-Arellano, Alicia; Madrigal-Bujaidar, Eduardo. 2009. "Investigation on the Protective Effect of α-Mannan against the DNA Damage Induced by Aflatoxin B1 in Mouse Hepatocytes." Int. J. Mol. Sci. 10, no. 2: 395-406.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert