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Int. J. Mol. Sci. 2009, 10(12), 5442-5454; doi:10.3390/ijms10125442

The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice

1
Department of Pediatrics, Renming Hospital, Wuhan University, Wuhan 430044, China
2
Department of Biochemistry and Molecular Biology, Tongji Medical College, Huazhong University of Science & Technology, Wuhan 430030, China
*
Author to whom correspondence should be addressed.
Received: 9 October 2009 / Accepted: 17 December 2009 / Published: 18 December 2009
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
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Abstract

Objective: To demonstrate the inhibitory function of the prodomain of tumor necrosis factor-α (TNF-α) converting enzyme (TACE) on TACE activity and to develop an approach to interfere with inflammation processes. Methods: The cDNA encoding the fulllength ectodomain (T1300) and prodomain (T591) of TACE were amplified by RT-PCR. The expression plasmids (pET-28a (+)-T1300 and pET-28a (+)-T591) were constructed and transformed into E. coli BL21. After Ni2+-NTA resin affinity chromatography, the recombinant T591 protein was obtained and assayed. In order to detect its inhibiton of TACE activity, the mice in the LPS-induced endotoxemia model group were treated with the recombinant TACE prodomain protein prior to the injection of LPS. Murine peritoneal macrophages were isolated from mice abdominal cavity for FCM and the liver, kidney and lung were removed for traditionally histopathology sectioning. Results: The FCM results showed that the recombinant prodomain protein decreased the release of the sTNF-α, which mediated the accumulation of TNF-α on the surface of macrophage cells. HE staining proved that the recombinant protein can decrease the inflammatory response in internal organs of endotoxaemia mice. Conclusions: The recombinant prodomain of TACE has the ability to inhibit sTNF-α release, which indicates that prodomain is an effective antagonist of TACE and might be useful in the molecular design of anti-inflammatory drugs.
Keywords: TACE; TNF-α; prodomain; inhibitor TACE; TNF-α; prodomain; inhibitor
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Li, X.; Yan, Y.; Huang, W.; Yang, Y. The Study of the Inhibition of the Recombinant TACE Prodomain to Endotoxemia in Mice. Int. J. Mol. Sci. 2009, 10, 5442-5454.

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