Int. J. Mol. Sci. 2009, 10(11), 4963-4976; doi:10.3390/ijms10114963
Article

Heat Shock-Induced Three-Dimensional-Like Proliferation of Normal Human Fibroblasts Mediated by Pressed Silk

Fukumi Hiragami 1,* email, Hirotoshi Motoda 1 email, Toshiaki Takezawa 2 email, Chiyuki Takabayashi 3 email, Shigeki Inoue 1 email, Yuji Wakatake 1 email and Yoshio Kano 4 email
1 Department of Physical Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan 2 Laboratory of Animal Cell Biology, National Institute of Agrobiological Sciences, Ikenodai 2, Tsukuba, Ibaraki 305-0901, Japan 3 Laboratory of New Silk Materials, National Institute of Agrobiological Sciences, Gouda 1-4-8, Okaya, Nagano 394-0021, Japan 4 Department of Occupational Therapy, School of Health Science, Kibi International University, 8 Iga-machi Takahashi City, Okayama 716-8505, Japan
* Author to whom correspondence should be addressed.
Received: 30 September 2009 / Accepted: 30 October 2009 / Published: 12 November 2009
(This article belongs to the Special Issue Biocompatibility of Materials)
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Abstract: The aim of this study was to determine the optimal heat treatment conditions for enhancement of pressed silk-mediated 3D-like proliferation of normal human dermal fibroblasts, as well as to determine the responses to heat shock of cells and intracellular signaling pathways. The beginning of 3D-like pattern formation of cells was observed in the second week after the start of the experiment. The mean rates of beginning of 3D-like pattern formation by cells heat-treated at 40 ºC and 43 ºC for 10 min were significantly higher (3.2- and 8.6-fold, respectively) than that of untreated cells. We found that apoptosis had occurred in 7.5% and 50.0% of the cells at one week after heat treatment for 10 min at 43 ºC and 45 ºC, respectively. Western blot analysis demonstrated that phosphorylation of p38 MAPK and that of Hsp27 were markedly increased by heat treatment at 43 ºC for 10 min. The results of an experiment using a p38 MAPK inhibitor and Hsp27 inhibitor suggest that activation of p38 MAPK by heat shock is associated with 3D-like cell proliferation and that Hsp27 contributes to the inhibition of apoptosis. The results of this study should be useful for further studies aimed at elucidation of the physiologic mechanisms underlying thermotherapy.
Keywords: normal human dermal fibroblasts; heat treatment; three-dimensional-like proliferation patterns; p38 MAPK; Hsp27

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Cite This Article

MDPI and ACS Style

Hiragami, F.; Motoda, H.; Takezawa, T.; Takabayashi, C.; Inoue, S.; Wakatake, Y.; Kano, Y. Heat Shock-Induced Three-Dimensional-Like Proliferation of Normal Human Fibroblasts Mediated by Pressed Silk. Int. J. Mol. Sci. 2009, 10, 4963-4976.

AMA Style

Hiragami F, Motoda H, Takezawa T, Takabayashi C, Inoue S, Wakatake Y, Kano Y. Heat Shock-Induced Three-Dimensional-Like Proliferation of Normal Human Fibroblasts Mediated by Pressed Silk. International Journal of Molecular Sciences. 2009; 10(11):4963-4976.

Chicago/Turabian Style

Hiragami, Fukumi; Motoda, Hirotoshi; Takezawa, Toshiaki; Takabayashi, Chiyuki; Inoue, Shigeki; Wakatake, Yuji; Kano, Yoshio. 2009. "Heat Shock-Induced Three-Dimensional-Like Proliferation of Normal Human Fibroblasts Mediated by Pressed Silk." Int. J. Mol. Sci. 10, no. 11: 4963-4976.

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