Effect of Peptide-Conjugated Near-Infrared Fluorescent Quantum Dots (NIRF-QDs) on the Invasion and Metastasis of Human Tongue Squamous Cell Carcinoma Cell Line Tca8113 in Vitro
Abstract
:1. Introduction
2. Results and Discussion
2.1. Results
2.1.1. Labeling of Tca8113 Cells with QDs
2.1.2. Effect of QDs on the Proliferation of Tca8113 Cells
2.1.3. Effect of QDs on the Tca8113 Cell Clone Formation
2.1.4. Effect of QDs on the Tca8113 Cell Adhesion
2.1.5. Effect of QDs on the Tca8113 Cell Invasion
2.1.6. Effect of QDs on the Tca8113 Cell Chemotaxis
2.2. Discussion
3. Experimental Section
3.1. Materials and Instruments
3.2. Labeling Tca8113 Cells with QDs
3.3. In Vitro Cell Growth Assay
3.4. Plate Clone Formation Assay
3.5. Detection of Cell Adherence
3.6. Detection of Cell Invasion
3.7. Cell Chemotaxis Assay
4. Conclusions
Acknowledgments
References
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Time(min) | Adhesion rate (%) | P value(χ2 test) | |
---|---|---|---|
Tca8113/QD800 | Tca8113 | ||
30 | 10.3 ± 1.5 | 10.7 ± 1.6 | >0.05 |
60 | 16.5 ± 2.4 | 15.9 ± 2.8 | >0.05 |
90 | 27.2 ± 3.4 | 30.1 ± 2.9 | >0.05 |
120 | 39.5 ± 3.6 | 40.8 ± 3.8 | >0.05 |
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Yang, K.; Li, Z.; Cao, Y.; Yu, X.; Mei, J. Effect of Peptide-Conjugated Near-Infrared Fluorescent Quantum Dots (NIRF-QDs) on the Invasion and Metastasis of Human Tongue Squamous Cell Carcinoma Cell Line Tca8113 in Vitro. Int. J. Mol. Sci. 2009, 10, 4418-4427. https://doi.org/10.3390/ijms10104418
Yang K, Li Z, Cao Y, Yu X, Mei J. Effect of Peptide-Conjugated Near-Infrared Fluorescent Quantum Dots (NIRF-QDs) on the Invasion and Metastasis of Human Tongue Squamous Cell Carcinoma Cell Line Tca8113 in Vitro. International Journal of Molecular Sciences. 2009; 10(10):4418-4427. https://doi.org/10.3390/ijms10104418
Chicago/Turabian StyleYang, Kai, Zhigang Li, Yuan Cao, Xiuli Yu, and Jie Mei. 2009. "Effect of Peptide-Conjugated Near-Infrared Fluorescent Quantum Dots (NIRF-QDs) on the Invasion and Metastasis of Human Tongue Squamous Cell Carcinoma Cell Line Tca8113 in Vitro" International Journal of Molecular Sciences 10, no. 10: 4418-4427. https://doi.org/10.3390/ijms10104418