Int. J. Mol. Sci. 2009, 10(1), 18-27; doi:10.3390/ijms10010018
Article

Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)

1 Aquaculture Genetics and Breeding Laboratory, Ohio State University Aquaculture Research and Development Integration Program, 1864 Shyville Road, Piketon, Ohio 45661, USA 2 Department of Chemical & Biomolecular Engineering, Ohio State University, 140 West 19th Ave., Columbus, Ohio 43210, USA 3 Ecological Genetics Laboratory, Virginia Commonwealth University, Richmond, Virginia 23284, USA
* Author to whom correspondence should be addressed.
Received: 27 November 2008; in revised form: 15 December 2008 / Accepted: 18 December 2008 / Published: 27 December 2008
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
PDF Full-text Download PDF Full-Text [120 KB, uploaded 27 December 2008 18:07 CET]
Abstract: To perform whole genome scanning for complex trait analysis, we isolated and characterized a total of 21 novel genomic-SSRs and EST-SSRs for yellow perch (Perca flavescens), using the methods of construction of SSR-enrichment libraries and EST database mining of a related species P. fluviatilis. Of 16 genomic-SSR primer pairs examined, eight successfully amplified scorable products. The number of alleles at these informative loci varied from 3 - 14 with an average of 8.5 alleles per locus. When tested on wild perch from a population in Pennsylvania, observed and expected heterozygosities ranged from 0.07 - 0.81 and from 0.37 - 0.95, respectively. Of 2,226 EST sequences examined, only 110 (4.93%) contained microsatellites and for those, 13 markers were tested, 12 of which exhibited polymorphism. Compared with genomic-SSRs, EST-SSRs exhibited a lower level of genetic variability with the number of alleles of averaging only 2.6 alleles per locus. Cross-species utility indicated that three of the genomic-SSRs and eight of the EST-SSRs successfully cross-amplified in a related species, the walleye (Sander vitreus).
Keywords: Microsatellite; genomic-SSRs; EST-SSRs; yellow perch Perca flavescens

Article Statistics

Load and display the download statistics.

Citations to this Article

Cite This Article

MDPI and ACS Style

Zhan, A.; Wang, Y.; Brown, B.; Wang, H.-P. Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens). Int. J. Mol. Sci. 2009, 10, 18-27.

AMA Style

Zhan A, Wang Y, Brown B, Wang H-P. Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens). International Journal of Molecular Sciences. 2009; 10(1):18-27.

Chicago/Turabian Style

Zhan, Aibin; Wang, Yao; Brown, Bonnie; Wang, Han-Ping. 2009. "Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)." Int. J. Mol. Sci. 10, no. 1: 18-27.

Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert