Next Article in Journal
Previous Article in Journal
Int. J. Mol. Sci. 2009, 10(1), 18-27; doi:10.3390/ijms10010018
Article

Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)

1
, 2
, 3
 and 1,*
Received: 27 November 2008; in revised form: 15 December 2008 / Accepted: 18 December 2008 / Published: 27 December 2008
(This article belongs to the Section Biochemistry, Molecular Biology and Biophysics)
View Full-Text   |   Download PDF [120 KB, uploaded 19 June 2014]
Abstract: To perform whole genome scanning for complex trait analysis, we isolated and characterized a total of 21 novel genomic-SSRs and EST-SSRs for yellow perch (Perca flavescens), using the methods of construction of SSR-enrichment libraries and EST database mining of a related species P. fluviatilis. Of 16 genomic-SSR primer pairs examined, eight successfully amplified scorable products. The number of alleles at these informative loci varied from 3 - 14 with an average of 8.5 alleles per locus. When tested on wild perch from a population in Pennsylvania, observed and expected heterozygosities ranged from 0.07 - 0.81 and from 0.37 - 0.95, respectively. Of 2,226 EST sequences examined, only 110 (4.93%) contained microsatellites and for those, 13 markers were tested, 12 of which exhibited polymorphism. Compared with genomic-SSRs, EST-SSRs exhibited a lower level of genetic variability with the number of alleles of averaging only 2.6 alleles per locus. Cross-species utility indicated that three of the genomic-SSRs and eight of the EST-SSRs successfully cross-amplified in a related species, the walleye (Sander vitreus).
Keywords: Microsatellite; genomic-SSRs; EST-SSRs; yellow perch Perca flavescens Microsatellite; genomic-SSRs; EST-SSRs; yellow perch Perca flavescens
This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

Export to BibTeX |
EndNote


MDPI and ACS Style

Zhan, A.; Wang, Y.; Brown, B.; Wang, H.-P. Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens). Int. J. Mol. Sci. 2009, 10, 18-27.

AMA Style

Zhan A, Wang Y, Brown B, Wang H-P. Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens). International Journal of Molecular Sciences. 2009; 10(1):18-27.

Chicago/Turabian Style

Zhan, Aibin; Wang, Yao; Brown, Bonnie; Wang, Han-Ping. 2009. "Isolation and Characterization of Novel Microsatellite Markers for Yellow Perch (Perca flavescens)." Int. J. Mol. Sci. 10, no. 1: 18-27.



Int. J. Mol. Sci. EISSN 1422-0067 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert