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Probing the Structure of DNA Aptamers with a Classic Heterocycle.
Department of Chemistry and Biochemistry, Mississippi College, 200 South Capitol St., Clinton, MS 39058. USA
* Author to whom correspondence should be addressed.
Received: 5 February 2004; in revised form: 9 February 2004 / Accepted: 15 February 2004 / Published: 28 February 2004
Abstract: DNA aptamers are synthetic, single-stranded DNA oligonucleotides selectedby SELEX methods for their binding with specific ligands. Here we present ethidiumbinding results for three related DNA aptamers (PDB code: 1OLD, 1DB6, and 2ARG)that bind L-argininamide (L-Arm). The ligand bound form of each aptamer's structurehas been reported and each are found to be composed primarily of two domainsconsisting of a stem helical region and a loop domain that forms a binding pocket for thecognate ligand. Previous thermodynamic experiments demonstrated that the DNAaptamer 1OLD undergoes a large conformational ordering upon binding to L-Arm. Herewe extend those linkage binding studies by examining the binding of the heterocyclicintercalator ethidium to each of the three aptamers by fluorescence and absorptionspectrophotometric titrations. Our results reveal that ethidium binds to each aptamer with∆Go's in the range of -8.7 to -9.4 kcal/mol. The stoichiometry of binding is 2:1 for eachaptamer and is quantitatively diminished in the presence of L-Arm as is the overallfluorescence intensity of ethidium. Together, these results demonstrate that a portion ofthe bound ethidium is excluded from the aptamer in the presence of a saturating amountof L-Arm. These results demonstrate the utility of ethidium and related compounds forthe probing of non-conventional DNA structures and reveal an interesting fundamentalthermodynamic linkage in DNA aptamers. Results are discussed in the context of thethermodynamic stability and structure of each of the aptamers examined.
Keywords: Ethidium; intercalator; DNA; aptamer; thermodynamics; linkage; fluorescence; free energy; Job plot
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Wood IV, A.E.; Bishop, G.R. Probing the Structure of DNA Aptamers with a Classic Heterocycle.. Molecules 2004, 9, 67-85.
Wood IV AE, Bishop GR. Probing the Structure of DNA Aptamers with a Classic Heterocycle.. Molecules. 2004; 9(3):67-85.
Wood IV, Arthur E.; Bishop, G. R. 2004. "Probing the Structure of DNA Aptamers with a Classic Heterocycle.." Molecules 9, no. 3: 67-85.