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A Simple Enzymatic Preparation of 2’,3’-Di-OAcetylnucleosides Through a Lipase Catalyzed Alcoholysis
Molecules 2000, 5(3), 535-536; doi:10.3390/50300535

Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides

1, 1, 1, 1,*  and 1,2
1 Universidad Nacional de Quilmes. R. S. Peña 180, (1876) Bernal, Buenos Aires, Argentina 2 INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
* Author to whom correspondence should be addressed.
Published: 22 March 2000
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E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.
This is an open access article distributed under the Creative Commons Attribution License (CC BY) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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Rogert, M.C.; Martínez, N.; Porro, S.; Lewkowicz, E.; Iribarren, A. Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides. Molecules 2000, 5, 535-536.

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