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A Simple Enzymatic Preparation of 2’,3’-Di-OAcetylnucleosides Through a Lipase Catalyzed Alcoholysis
Molecules 2000, 5(3), 535-536; doi:10.3390/50300535

Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides

1, 1, 1, 1,*  and 1,2
1 Universidad Nacional de Quilmes. R. S. Peña 180, (1876) Bernal, Buenos Aires, Argentina 2 INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina
* Author to whom correspondence should be addressed.
Published: 22 March 2000
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E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.
This is an open access article distributed under the Creative Commons Attribution License (CC BY 3.0).

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Rogert, M.C.; Martínez, N.; Porro, S.; Lewkowicz, E.; Iribarren, A. Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides. Molecules 2000, 5, 535-536.

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