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• Rogert, MC.
• Martínez, N
• Porro, S
• Lewkowicz, E
• Iribarren, A
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• Rogert, MC.
• Martínez, N
• Porro, S
• Lewkowicz, E
• Iribarren, A
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• Rogert, MC.
• Martínez, N
• Porro, S
• Lewkowicz, E
• Iribarren, A

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Molecules 2000, 5(3), 535-536; doi:10.3390/50300535

Article

Escherichia Coli Bl21: A Useful Biocatalyst for the Synthesis of Purine Nucleosides

M. C. Rogert ¹ , N. Martínez ¹ , S. Porro ¹ , E. Lewkowicz ^1,*  and A. Iribarren ^1,2

¹ Universidad Nacional de Quilmes. R. S. Peña 180, (1876) Bernal, Buenos Aires, Argentina ² INGEBI, CONICET, Vuelta de Obligado 2490, (1428) Buenos Aires, Argentina

* Author to whom correspondence should be addressed.

Published: 22 March 2000

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Abstract: E. coli BL21 cells were able to synthesize several purine nucleosides from pyrimidine ones. Kinetics and yields of this reaction showed a strong dependence on pH, temperature, reagent concentrations and weight of wet cell paste. Yields over 90% were reached in the synthesis of adenosine.

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