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Molecules 2018, 23(8), 1944; https://doi.org/10.3390/molecules23081944

Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch

Department of Bioprocess Technology, Faculty of Biotechnology and Biomolecular Sciences, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
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Author to whom correspondence should be addressed.
Academic Editors: Giancarlo Cravotto and Andrioletti Bruno
Received: 13 May 2018 / Revised: 26 June 2018 / Accepted: 26 June 2018 / Published: 3 August 2018
(This article belongs to the Special Issue Green and Sustainable Solvents)
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Abstract

This study was conducted in order to optimise simultaneous saccharification and fermentation (SSF) for biobutanol production from a pretreated oil palm empty fruit bunch (OPEFB) by Clostridium acetobutylicum ATCC 824. Temperature, initial pH, cellulase loading and substrate concentration were screened using one factor at a time (OFAT) and further statistically optimised by central composite design (CCD) using the response surface methodology (RSM) approach. Approximately 2.47 g/L of biobutanol concentration and 0.10 g/g of biobutanol yield were obtained after being screened through OFAT with 29.55% increment (1.42 fold). The optimised conditions for SSF after CCD were: temperature of 35 °C, initial pH of 5.5, cellulase loading of 15 FPU/g-substrate and substrate concentration of 5% (w/v). This optimisation study resulted in 55.95% increment (2.14 fold) of biobutanol concentration equivalent to 3.97 g/L and biobutanol yield of 0.16 g/g. The model and optimisation design obtained from this study are important for further improvement of biobutanol production, especially in consolidated bioprocessing technology. View Full-Text
Keywords: simultaneous saccharification and fermentation (SSF); oil palm empty fruit bunch (OPEFB); biobutanol; Clostridium acetobutylicum ATCC 824; optimisation simultaneous saccharification and fermentation (SSF); oil palm empty fruit bunch (OPEFB); biobutanol; Clostridium acetobutylicum ATCC 824; optimisation
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Md Razali, N.A.A.; Ibrahim, M.F.; Kamal Bahrin, E.; Abd-Aziz, S. Optimisation of Simultaneous Saccharification and Fermentation (SSF) for Biobutanol Production Using Pretreated Oil Palm Empty Fruit Bunch. Molecules 2018, 23, 1944.

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