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Molecules 2018, 23(7), 1687; https://doi.org/10.3390/molecules23071687

N-Butanol Subfraction of Brassica Rapa L. Promotes Reactive Oxygen Species Production and Induces Apoptosis of A549 Lung Adenocarcinoma Cells via Mitochondria-Dependent Pathway

1
Xinjiang Key Laboratory of Biological Resources and Genetic Engineering, College of Life Science and Technology, Xinjiang University, Urumqi 830046, Xinjiang, China
2
College of Life Science, Xinjiang Normal University, Urumqi 830054, Xinjiang, China
3
Key Laboratory of Medical Molecular Virology of MOE/MOH, Shanghai Medical College, Fudan University, 130 Dong An Road, Shanghai 200032, China
4
School of Science, and School of Interprofessional Health Studies, Faculty of Health & Environmental Sciences, Auckland University of Technology, Private Bag 92006, Auckland 1142, New Zealand
*
Authors to whom correspondence should be addressed.
Academic Editors: Zhe-Sheng (Jason) Chen and Dong-Hua Yang
Received: 21 May 2018 / Revised: 3 July 2018 / Accepted: 8 July 2018 / Published: 11 July 2018
(This article belongs to the Collection Natural Products: Anticancer Potential and Beyond)
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Abstract

Brassica rapa L., an edible and medical vegetable, has been traditionally used in Uyghur folk medicine to treat coughs and asthma in the Xinjiang Uygur Autonomous Region, China. In this study, we prepared an n-butanol subfraction of B. rapa L. (BRBS) and investigated the anti-tumor effect on A549 lung adenocarcinoma cells. The proliferation of A549 cells was significantly inhibited by BRBS treatment in a dose- and time-dependent manner. BRBS significantly induced cell cycle arrest and apoptosis in A549 cells through increased reactive oxygen species (ROS) production and mitochondrial dysfunction characterized by a reduction in mitochondrial membrane potential and the release of cytochrome c, which promoted caspase-3 and poly(ADP-ribose) polymerase processing. Moreover, BRBS significantly suppressed the migration of A549 cells in vitro. These results suggest that BRBS inhibited A549 cell proliferation through increased ROS production and the mitochondria-dependent apoptosis pathway. Consequently, BRBS might be a potential candidate for the treatment of lung cancer. View Full-Text
Keywords: Brassica rapa L.; antitumor; reactive oxygen species; apoptosis; cell cycle arrest; migration Brassica rapa L.; antitumor; reactive oxygen species; apoptosis; cell cycle arrest; migration
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Aipire, A.; Chen, Q.; Cai, S.; Li, J.; Fu, C.; Ying, T.; Lu, J.; Li, J. N-Butanol Subfraction of Brassica Rapa L. Promotes Reactive Oxygen Species Production and Induces Apoptosis of A549 Lung Adenocarcinoma Cells via Mitochondria-Dependent Pathway. Molecules 2018, 23, 1687.

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