Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea
Abstract
:1. Introduction
2. Results and Discussion
2.1. Isolation and Sequence Analysis of MpDBR and MpMDBRL
2.2. Sequence Alignment and Phylogenetic Analysis
2.3. Functional Analysis
2.4. Transcript Abundance of DBRs and Their Response to Phytohormone Treatment
3. Materials and Methods
3.1. Chemicals and Reagents
3.2. The Isolation and Analysis of DBR Sequences
3.3. Sequence Alignment and Phylogenetic Analysis
3.4. Recombinant Protein Expression and Purification
3.5. Enzyme Assays
3.6. Plant Phytohormone Treatment and Gene Expression Profiling
3.7. Sequence Accession Numbers
4. Conclusions
Supplementary Materials
Author Contributions
Acknowledgments
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are available from the authors. |
Substrate | Structure | Specific Activity (nmol mg−1 min−1) | |
---|---|---|---|
MpDBR | MpMDBRL | ||
p-Coumaryl aldehyde | 29.08 ± 1.48 | 21.26 ± 1.46 | |
Caffeyl aldehyde | 29.60 ± 1.35 | 25.88 ± 0.88 | |
Coniferyl aldehyde | 28.61 ± 0.69 | 20.17 ± 0.48 | |
5-Hydroxyconiferyl aldehyde | 26.87 ± 2.21 | 21.34 ± 0.54 | |
Sinapyl aldehyde | ND a | ND |
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Wu, Y.-F.; Zheng, H.-B.; Liu, X.-Y.; Cheng, A.-X.; Lou, H.-X. Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea. Molecules 2018, 23, 1630. https://doi.org/10.3390/molecules23071630
Wu Y-F, Zheng H-B, Liu X-Y, Cheng A-X, Lou H-X. Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea. Molecules. 2018; 23(7):1630. https://doi.org/10.3390/molecules23071630
Chicago/Turabian StyleWu, Yi-Feng, Hong-Bo Zheng, Xin-Yan Liu, Ai-Xia Cheng, and Hong-Xiang Lou. 2018. "Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea" Molecules 23, no. 7: 1630. https://doi.org/10.3390/molecules23071630