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Molecules 2018, 23(7), 1630; https://doi.org/10.3390/molecules23071630

Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea

Key Laboratory of Chemical Biology of Natural Products, Ministry of Education, School of Pharmaceutical Sciences, Shandong University, Jinan 250012, China
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Received: 14 June 2015 / Revised: 2 July 2018 / Accepted: 3 July 2018 / Published: 4 July 2018
(This article belongs to the Section Chemical Biology)
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Abstract

Alkenal double bond reductases (DBRs), capable of catalyzing the NADPH-dependent reduction of the α,β-unsaturated double bond, play key roles in the detoxication of alkenal carbonyls. Here, the isolation and characterization of two DBRs encoded by the liverwort species Marchantia paleacea are described. The two DBRs share a relatively low similarity, and phylogenetic analysis indicated that MpMDBRL is more closely related to microbial DBRs than to other plant DBRs, while MpDBR shares common ancestry with typical plant DBRs. Both DBR proteins exhibited hydrogenation ability towards hydroxycinnamyl aldehydes; however, their temperature optimums were strikingly different. MpMDBRL demonstrated slightly weaker catalytic efficiency compared to MpDBR, and the structural models of their active binding sites to the substrate may provide a parsimonious explanation. Furthermore, both DBRs significantly responded to phytohormone treatment. In conclusion, M. paleacea produces two distinct types of functional DBRs, both of which participate in the protection against environmental stress in liverwort. The presence of a microbial type of DBR in a plant is herein reported for the first time. View Full-Text
Keywords: alkenal double bond reductase; Marchantia paleacea; hydroxycinnamyl aldehydes; microbial type; expression pattern alkenal double bond reductase; Marchantia paleacea; hydroxycinnamyl aldehydes; microbial type; expression pattern
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wu, Y.-F.; Zheng, H.-B.; Liu, X.-Y.; Cheng, A.-X.; Lou, H.-X. Molecular Diversity of Alkenal Double Bond Reductases in the Liverwort Marchantia paleacea. Molecules 2018, 23, 1630.

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