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Molecules 2018, 23(4), 984; https://doi.org/10.3390/molecules23040984

Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies

1
Research Institute of Pharmaceutical Sciences, College of Pharmacy, Kyungpook National University, Daegu 41566, Korea
2
Department of Clinical Pharmacology, Konkuk University Medical Center, Seoul 05029, Korea
*
Author to whom correspondence should be addressed.
Academic Editors: Nicola Marchetti and Anna Laura Capriotti
Received: 28 February 2018 / Revised: 15 April 2018 / Accepted: 19 April 2018 / Published: 23 April 2018
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Abstract

Green tea is consumed as a beverage worldwide and has beneficial effects, such as a lower risk of cardiovascular disease and cancer. A quantitative analysis of the beneficial components in plasma is important for understanding the potential health benefits of green tea. Four catechins—epigallocatechin-3-gallate (EGCG), epicatechin-3-gallate (ECG), epigallocatechin (EGC), and epicatechin (EC)—which account for the majority of the components of green tea, were analyzed by ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS). In this study, a validated method was optimized to obtain the blood concentrations after the one-time ingestion of 630 mg green tea extract with digoxin and then after the ingestion of 630 mg green tea repeatedly for 15 days. The calibration curve, including the LLOQ, was constructed over 1–500 ng/mL for EGCG, ECG, and EGC and 0.1–50 ng/mL for EC. The method for inter- and intra-validation was applied, acceptable for both accuracy and precision. We successfully developed an appropriate UPLC-MS/MS method for human plasma with good reproducibility and sensitivity. Thus, this method could be applied for future preclinical and clinical studies on EGCG, ECG, EGC, and EC. View Full-Text
Keywords: green tea; epigallocatechin-3-gallate (EGCG); epicatechin-3-gallate (ECG); epigallocatechin (EGC); epicatechin (EC); ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS) green tea; epigallocatechin-3-gallate (EGCG); epicatechin-3-gallate (ECG); epigallocatechin (EGC); epicatechin (EC); ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS/MS)
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).
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Park, J.-E.; Kim, T.-E.; Shin, K.-H. Quantitative Analysis of Four Catechins from Green Tea Extract in Human Plasma Using Ultra-Performance Liquid Chromatography-Tandem Mass Spectrometry for Pharmacokinetic Studies. Molecules 2018, 23, 984.

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