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Molecules 2018, 23(1), 137; doi:10.3390/molecules23010137

Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01

Research and Development Center of Biorational Pesticide, Northwest Agriculture & Forestry University, Yangling 712100, China
Agriculture Research Institute, Tibet Academy of Agricultural and Animal Husbandry Science, Lhasa 850032, China
Shaanxi Research Center of Biopesticide Engineering & Technology, Yangling 712100, China
Authors to whom correspondence should be addressed.
Received: 18 December 2017 / Revised: 8 January 2018 / Accepted: 9 January 2018 / Published: 10 January 2018
(This article belongs to the Section Metabolites)
View Full-Text   |   Download PDF [2709 KB, uploaded 10 January 2018]   |  


GP-1 is a novel glycoprotein produced by Streptomyces kanasenisi ZX01 that was isolated from soil near Kanas Lake with significant bioactivity against tobacco mosaic virus. However, extremely low fermentation production has largely hindered further research and market applications on glycoprotein GP-1. In this study, response surface methodology was used to optimize fermentation conditions in a shake flask for higher glycoprotein GP-1 production. When the optimized fermentation conditions were inoculum volume of 6%, initial pH of 6.5, and rotating speed of 150 rpm, glycoprotein GP-1 production could reach 0.9253 mg/L, which was increased by 52.14% compared to the original conditions. In addition, scale-up fermentation was conducted in a 5-L bioreactor to preliminarily explore the feasibility for mass production of glycoprotein GP-1 in a large fermentor, obtaining GP-1 production of 2.54 mg/L under the same conditions, which was 2.75 times higher than the production obtained from a shake flask of 0.9253 mg/L. This work will be helpful to improve GP-1 production on a large scale and lay the foundations for developing it as a novel agent against plant virus. View Full-Text
Keywords: response surface methodology; glycoprotein; Streptomyces; anti-TMV; optimization response surface methodology; glycoprotein; Streptomyces; anti-TMV; optimization

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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Zhou, Y.; Zhou, X.; Yu, D.-L.; Sang, B.; Feng, J.-T.; Han, L.-R.; Zhang, X. Optimization of Fermentation Conditions and Bench-Scale for Improvement of a Novel Glycoprotein GP-1 Production by Streptomyces kanasenisi ZX01. Molecules 2018, 23, 137.

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