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Molecules 2017, 22(9), 1460; doi:10.3390/molecules22091460

Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications

Department of Microbiology and Microbial Biotechnology, Faculty of Biological Sciences and Technology, University of Shahid-Beheshty, Tehran 1983963113, Iran
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Received: 22 July 2017 / Revised: 25 August 2017 / Accepted: 31 August 2017 / Published: 11 September 2017
(This article belongs to the Special Issue Lipases and Lipases Modification)
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Abstract

The Plackett–Burman design and the Box–Behnken design, statistical methodologies, were employed for the optimization lipase and biosurfactant production by Ochrobactrum intermedium strain MZV101 in an identical broth medium for detergent applications. Environmental factor pH determined to be most mutual significant variables on production. A high concentration of molasses at high temperature and pH has a negative effect on lipase and biosurfactant production by O. intermedium strain MZV101. The chosen mathematical method of medium optimization was sufficient for improving the industrial production of lipase and biosurfactant by bacteria, which were respectively increased 3.46- and 1.89-fold. The duration of maximum production became 24 h shorter, so it was fast and cost-saving. In conclusion, lipase and biosurfactant production by O. intermedium strain MZV101 in an identical culture medium at pH 10.5–11 and 50–60 °C, with 1 g/L of molasses, seemed to be economical, fast, and effective for the enhancement of yield percentage for use in detergent applications. View Full-Text
Keywords: Ochrobactrum intermedium strain MZV101; lipase; biosurfactant; detergent Ochrobactrum intermedium strain MZV101; lipase; biosurfactant; detergent
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Ebrahimipour, G.; Sadeghi, H.; Zarinviarsagh, M. Statistical Methodologies for the Optimization of Lipase and Biosurfactant by Ochrobactrum intermedium Strain MZV101 in an Identical Medium for Detergent Applications. Molecules 2017, 22, 1460.

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