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Molecules 2017, 22(8), 1364; doi:10.3390/molecules22081364

Biochemical and Comparative Transcriptomic Analyses Identify Candidate Genes Related to Variegation Formation in Paeonia rockii

1,2,†
,
3,†
,
1,2,†
,
1,2
,
1,2
,
1,2
,
4,5
and
1,2,*
1
College of Landscape Architecture and Art, Northwest A&F University, Yangling 712100, Shaanxi, China
2
National Engineering Research Center for Oil Peony, Yangling 712100, Shaanxi, China
3
College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China
4
Gansu Engineering Research Center for Tree Peony, Lanzhou 730046, Gansu, China
5
Gansu Forestry Science and Technology Extend Station, Lanzhou 730046, Gansu, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 16 July 2017 / Accepted: 14 August 2017 / Published: 17 August 2017
View Full-Text   |   Download PDF [17205 KB, uploaded 21 August 2017]   |  

Abstract

Paeonia rockii is a wild tree peony species with large and dark purple variegations at the base of its petals. It is the genetic resource for various variegation patterns in tree peony cultivars, which is in contrast to the pure white petals of Paeonia ostii. However, the molecular mechanism underlying the formation of variegation in this plant is still unknown. Here, we conducted Illumina transcriptome sequencing for P. rockii, P. ostii (with pure white petals) and their F1 individuals (with purple-red variegation). A total of 181,866 unigenes were generated, including a variety of unigenes involved in anthocyanin biosynthesis and sequestration and the regulation of anthocyanin biosynthesis. The dark purple or purple-red variegation patterns mainly occurred due to the proportions of cyanidin (Cy)- and peonidin (Pn)-based anthocyanins. The variegations of P. rockii exhibited a “Cy > Pn” phenotype, whereas the F1 progeny showed a “Pn > Cy” phenotype. The CHS, DFR, ANS, and GST genes might play key roles in variegation pigmentation in P. rockii according to gene expression and interaction network analysis. Two R2R3-MYB transcription factors (c131300.graph_c0 and c133735.graph_c0) regulated variegation formation by controlling CHS, ANS and GST genes. Our results indicated that the various variegation patterns were caused by transcriptional regulation of anthocyanin biosynthesis genes, and the transcription profiles of the R2R3-MYBs provided clues to elucidate the mechanisms underlying this trait. The petal transcriptome data produced in this study will provide a valuable resource for future association investigations of the genetic regulation of various variegation patterns in tree peonies. View Full-Text
Keywords: anthocyanin biosynthesis; comparative transcriptomic; R2R3-MYB; P. rockii; variegation formation anthocyanin biosynthesis; comparative transcriptomic; R2R3-MYB; P. rockii; variegation formation
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MDPI and ACS Style

Shi, Q.; Li, L.; Zhang, X.; Luo, J.; Li, X.; Zhai, L.; He, L.; Zhang, Y. Biochemical and Comparative Transcriptomic Analyses Identify Candidate Genes Related to Variegation Formation in Paeonia rockii. Molecules 2017, 22, 1364.

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