A Unique and Simple Approach to Improve Sensitivity in 15N-NMR Relaxation Measurements for NH3+ Groups: Application to a Protein-DNA Complex
Abstract
:1. Introduction
2. Results
2.1. Previous and Current Approaches to Eliminating the Adverse Effects of Multi-Spin Order Terms
2.2. Assessment of the Sensitivity-Improved 15N R1 Experiment for NH3+ Groups
2.3. Assessment of the Sensitivity-Improved 15N R2 Experiment for NH3+ Groups
2.4. Assessment of the Sensitivity-Improved Heteronuclear NOE Experiment for NH3+ Groups
3. Discussion
4. Materials and Methods
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Sample Availability: Not available. |
Parameters | K46 | K55 | K57 | K58 |
---|---|---|---|---|
15N R1 (s−1) b | 1.093 ± 0.013 | 0.637 ± 0.005 | 1.035 ± 0.004 | 0.363 ± 0.002 |
15N R1 (s−1) c | 1.081 ± 0.023 | 0.617 ± 0.008 | 1.037 ± 0.008 | 0.364 ± 0.003 |
15N R2,ini (s−1) b | 2.55 ± 0.10 | 1.76 ± 0.07 | 2.95 ± 0.04 | 1.20 ± 0.03 |
15N R2,ini (s−1) c | 2.74 ± 0.20 | 2.05 ± 0.12 | 2.76 ± 0.06 | 1.14 ± 0.06 |
Heteronuclear NOE b | −2.44 ± 0.12 | −2.83 ± 0.10 | −2.54 ± 0.05 | −2.71 ± 0.05 |
Heteronuclear NOE c | −2.53 ± 0.18 | −2.75 ± 0.13 | −2.60 ± 0.08 | −2.65 ± 0.07 |
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Nguyen, D.; Lokesh, G.L.R.; Volk, D.E.; Iwahara, J. A Unique and Simple Approach to Improve Sensitivity in 15N-NMR Relaxation Measurements for NH3+ Groups: Application to a Protein-DNA Complex. Molecules 2017, 22, 1355. https://doi.org/10.3390/molecules22081355
Nguyen D, Lokesh GLR, Volk DE, Iwahara J. A Unique and Simple Approach to Improve Sensitivity in 15N-NMR Relaxation Measurements for NH3+ Groups: Application to a Protein-DNA Complex. Molecules. 2017; 22(8):1355. https://doi.org/10.3390/molecules22081355
Chicago/Turabian StyleNguyen, Dan, Ganesh L. R. Lokesh, David E. Volk, and Junji Iwahara. 2017. "A Unique and Simple Approach to Improve Sensitivity in 15N-NMR Relaxation Measurements for NH3+ Groups: Application to a Protein-DNA Complex" Molecules 22, no. 8: 1355. https://doi.org/10.3390/molecules22081355