Next Article in Journal
General Methodologies Toward cis-Fused Quinone Sesquiterpenoids. Enantiospecific Synthesis of the epi-Ilimaquinone Core Featuring Sc-Catalyzed Ring Expansion
Previous Article in Journal
Eu@C72: Computed Comparable Populations of Two Non-IPR Isomers
Article Menu
Issue 7 (July) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(7), 1055; doi:10.3390/molecules22071055

Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars

1
National Institute of Agricultural Science, RDA, Jeonju 54874, Korea
2
USDA-ARS, Western Regional Research Center, 800 Buchanan Street, Albany, CA 94710, USA
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Received: 7 June 2017 / Revised: 22 June 2017 / Accepted: 22 June 2017 / Published: 24 June 2017
View Full-Text   |   Download PDF [1294 KB, uploaded 24 June 2017]   |  

Abstract

The accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits. The method was used to verify the allelic compositions of 32 Korean wheat cultivars previously determined using SDS-PAGE and to assess the compositions of six new Korean cultivars. Three cultivars contained subunits that were identified incorrectly in the earlier analysis. The improved RP-HPLC method combined with conventional SDS-PAGE provides for accurate, efficient and reliable identification of HMW-GS and will contribute to efforts to improve wheat end-use quality. View Full-Text
Keywords: allelic analysis; high-molecular-weight glutenin subunit; RP-HPLC; SDS-PAGE allelic analysis; high-molecular-weight glutenin subunit; RP-HPLC; SDS-PAGE
Figures

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Jang, Y.-R.; Beom, H.-R.; Altenbach, S.B.; Lee, M.-K.; Lim, S.-H.; Lee, J.-Y. Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars. Molecules 2017, 22, 1055.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top