Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars
AbstractThe accurate identification of alleles for high-molecular weight glutenins (HMW-GS) is critical for wheat breeding programs targeting end-use quality. RP-HPLC methods were optimized for separation of HMW-GS, resulting in enhanced resolution of 1By and 1Dx subunits. Statistically significant differences in retention times (RTs) for subunits corresponding to HMW-GS alleles were determined using 16 standard wheat cultivars with known HMW-GS compositions. Subunits that were not identified unambiguously by RP-HPLC were distinguished by SDS-PAGE or inferred from association with linked subunits. The method was used to verify the allelic compositions of 32 Korean wheat cultivars previously determined using SDS-PAGE and to assess the compositions of six new Korean cultivars. Three cultivars contained subunits that were identified incorrectly in the earlier analysis. The improved RP-HPLC method combined with conventional SDS-PAGE provides for accurate, efficient and reliable identification of HMW-GS and will contribute to efforts to improve wheat end-use quality. View Full-Text
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Jang, Y.-R.; Beom, H.-R.; Altenbach, S.B.; Lee, M.-K.; Lim, S.-H.; Lee, J.-Y. Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars. Molecules 2017, 22, 1055.
Jang Y-R, Beom H-R, Altenbach SB, Lee M-K, Lim S-H, Lee J-Y. Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars. Molecules. 2017; 22(7):1055.Chicago/Turabian Style
Jang, You-Ran; Beom, Hye-Rang; Altenbach, Susan B.; Lee, Min-Ki; Lim, Sun-Hyung; Lee, Jong-Yeol. 2017. "Improved Method for Reliable HMW-GS Identification by RP-HPLC and SDS-PAGE in Common Wheat Cultivars." Molecules 22, no. 7: 1055.
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