Two New β-Dihydroagarofuran Sesquiterpenes from Celastrus orbiculatus Thunb and Their Anti-Proliferative Activity
Abstract
:1. Introduction
2. Results and Discussion
2.1. Two New Identified Compounds (1–2)
2.2. Anti-Proliferative Activity of Compounds 1–19 on HL-60, K562, and HCT116 Cell Lines
3. Experimental
3.1. General Experimental Procedures
3.2. Plant Material
3.3. Extraction and Isolation
3.4. Seventeen Known Compounds
3.5. Cell Culture
3.6. In Vitro Anti-Proliferative Bioassay
4. Conclusions
Supplementary Materials
Author Contributions
Conflicts of Interest
References
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Sample Availability: Samples of the compounds are not available from the authors. |
Position | 1 | 2 |
---|---|---|
1 | 5.58 (1H, dd, J = 12.4 Hz, 4.5 Hz) | 5.53 (1H, dd, J = 12.4 Hz, 4.3 Hz) |
2 | 1.86 (1H, m), 1.55 (1H, m) | 1.85 (1H, m), 1.55 (1H, m) |
3 | 2.25 (1H, m), 1.46 (1H, m) | 2.26 (1H, m), 1.45 (1H, m) |
4 | 2.29 (1H, m) | 1.86 (1H, m) |
5 | ||
6 | 5.92 (1H, s) | 2.21 (1H, m), 2.01 (1H, m) |
7 | 2.19 (1H, m) | 2.04 (1H, m) |
8 | 2.43 (1H, m), 2.15 (1H, m) | 2.19 (1H, m), 2.07 (1H, m) |
9 | 5.16 (1H, d, J = 7.3 Hz) | 5.20 (1H, d, J = 6.8 Hz) |
10 | ||
11 | ||
12 | 0.97 (3H, d, J = 7.4 Hz) | 1.08 (3H, d, J = 7.8 Hz) |
13 | 4.66 (1H, d, J = 12.2 Hz) 4.41 (1H, d, J = 12.3 Hz) | 4.52 (1H, d, J = 12.2 Hz) 4.47 (1H, d, J = 12.1 Hz) |
14 | 1.43 (3H, s) | 1.39 (3H, s) |
15 | 1.39 (3H, s) | 1.19 (3H, s) |
Position | 1 | 2 |
---|---|---|
1 | 73.1 | 73.4 |
2 | 22.5 | 22.8 |
3 | 26.5 | 26.8 |
4 | 33.5 | 40.1 |
5 | 89.7 | 87.0 |
6 | 78.2 | 36.7 |
7 | 48.8 | 43.6 |
8 | 34.7 | 33.9 |
9 | 69.8 | 69.9 |
10 | 53.0 | 50.6 |
11 | 82.5 | 82.0 |
12 | 16.6 | 17.4 |
13 | 65.5 | 65.0 |
14 | 25.9 | 24.4 |
15 | 30.5 | 30.3 |
Compounds | IC50 (μΜ) | ||
---|---|---|---|
HL-60 | K562 | HCT-116 | |
1 | - | - | - |
2 | 23.11 | 35.00 | 50.64 |
3 | 26.34 | 38.75 | 46.61 |
4 | 3.61 | 17.13 | 10.15 |
5 | 33.29 | 42.85 | 34.25 |
6 | 19.32 | 37.27 | 37.46 |
7 | 30.85 | 30.20 | - |
8 | - | - | - |
9 | - | - | 36.03 |
10 | 20.32 | 35.84 | 41.64 |
11 | - | - | - |
12 | - | - | - |
13 | 40.50 | 42.67 | - |
14 | - | - | 30.98 |
15 | 31.51 | 40.86 | 50.01 |
16 | 33.51 | 42.56 | - |
17 | 18.87 | 35.22 | - |
18 | - | - | - |
19 | 23.55 | 47.31 | 31.05 |
5-FU | 11.06 | 27.55 | 29.13 |
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Zhou, J.; Han, N.; Lv, G.; Jia, L.; Liu, Z.; Yin, J. Two New β-Dihydroagarofuran Sesquiterpenes from Celastrus orbiculatus Thunb and Their Anti-Proliferative Activity. Molecules 2017, 22, 948. https://doi.org/10.3390/molecules22060948
Zhou J, Han N, Lv G, Jia L, Liu Z, Yin J. Two New β-Dihydroagarofuran Sesquiterpenes from Celastrus orbiculatus Thunb and Their Anti-Proliferative Activity. Molecules. 2017; 22(6):948. https://doi.org/10.3390/molecules22060948
Chicago/Turabian StyleZhou, Jingjing, Na Han, Guanghui Lv, Lina Jia, Zhihui Liu, and Jun Yin. 2017. "Two New β-Dihydroagarofuran Sesquiterpenes from Celastrus orbiculatus Thunb and Their Anti-Proliferative Activity" Molecules 22, no. 6: 948. https://doi.org/10.3390/molecules22060948