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Molecules 2017, 22(6), 965; doi:10.3390/molecules22060965

Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction

1
College of Pharmacy, Chengdu University of Traditional Chinese Medicine, Chengdu 611137, China
2
Key Research Laboratory of Traditional Chinese Medicine Processing Technology, State Administration of Traditional Chinese Medicine of People’s Republic of China, Chengdu 611137, China
These authors contributed equally to this manuscript.
*
Author to whom correspondence should be addressed.
Academic Editors: Quan-Bin Han, Sunan Wang and Shaoping Nie
Received: 19 May 2017 / Revised: 5 June 2017 / Accepted: 7 June 2017 / Published: 9 June 2017
(This article belongs to the Special Issue Natural Polysaccharides)
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Abstract

In this study, the ultrasound-assisted extraction of polysaccharides (PSA) from Pinelliae Rhizoma Praeparatum Cum Alumine (PRPCA) was optimized by response surface methodology (RSM). The structural characteristics of PSA were analyzed by UV-vis spectroscopy, infrared spectroscopy, scanning electron microscopy, high performance gel permeation chromatography and high performance liquid chromatography, respectively. In addition, antioxidant and antimicrobial activities of PSA were studied by different in vitro assays. Results indicated that the optimal extraction conditions were as follows: the ratio of water to raw of 30 mL/g, extraction time of 46.50 min, ultrasonic temperature of 72.00 °C, and ultrasonic power of 230 W. Under these conditions, the obtained PSA yield (13.21 ± 0.37%) was closely agreed with the predicted yield by the model. The average molecular weights of the PSA were estimated to be 5.34 × 103 and 6.27 × 105 Da. Monosaccharide composition analysis indicated that PSA consisted of mannose, galactose uronic acid, glucose, galactose, arabinose with a molar ratio of 1.83:0.55:75.75:1.94:0.45. Furthermore, PSA exhibited moderate antioxidant and antibacterial activities in vitro. Collectively, this study provides a promising strategy to obtain bioactive polysaccharides from processed products of herbal medicines. View Full-Text
Keywords: polysaccharides; Pinelliae Rhizoma Praeparatum Cum Alumine; ultrasound-assisted extraction; response surface methodology; bioactivities polysaccharides; Pinelliae Rhizoma Praeparatum Cum Alumine; ultrasound-assisted extraction; response surface methodology; bioactivities
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Liu, Y.-J.; Mo, X.-L.; Tang, X.-Z.; Li, J.-H.; Hu, M.-B.; Yan, D.; Peng, W.; Wu, C.-J. Extraction Optimization, Characterization, and Bioactivities of Polysaccharides from Pinelliae Rhizoma Praeparatum Cum Alumine Employing Ultrasound-Assisted Extraction. Molecules 2017, 22, 965.

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