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Molecules 2017, 22(6), 932; doi:10.3390/molecules22060932

In Vitro Study of the Metabolic Characteristics of Eight Isoquinoline Alkaloids from Natural Plants in Rat Gut Microbiota

State Key Laboratory of Bioactive Substance and Function of Natural Medicines, Institute of Materia Medica, Chinese Academy of Medical Sciences/Peking Union Medical College, Beijing 100050, China
These authors contributed equally to this work.
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Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 29 April 2017 / Revised: 28 May 2017 / Accepted: 1 June 2017 / Published: 4 June 2017
(This article belongs to the Section Medicinal Chemistry)
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Abstract

Gut microbiota is populated with an immense number of microorganisms, which can be regulated by dietary components and drugs to markedly affect the nutritional and health status of the host. Eight medicinal isoquinoline alkaloids from natural plants were cultured anaerobically with rat gut microbiota and an LC/MSn-IT-TOF technique was used to identify the resulting metabolites. Palmatine, tetrahydropalmatine, dauricine, and tetrandrine containing nitro-hexatomic isoquinoline rings could be easily transformed by the intestinal flora in vitro and a total of nine demethylated metabolites were detected. However, sinomenine, homoharringtonine, harringtonine, and galanthamine, which all contained benzazepine, could not undergo demethylation. Computer-assisted docking was used to analyze the binding between these compounds and sterol 14α-demethylase. The computational results demonstrated that hydrophobic interactions were the main driving force for binding, but the steric hindrance produced by the benzazepine structure resulted in a weak interaction between the hit compounds and the enzyme. This work illustrated that gut microbiota were important in the metabolism of isoquinoline alkaloids. View Full-Text
Keywords: isoquinoline alkaloids; gut microbiota; metabolites; docking; 14α-demethylase; LC/MSn-IT-TOF isoquinoline alkaloids; gut microbiota; metabolites; docking; 14α-demethylase; LC/MSn-IT-TOF
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

He, C.-Y.; Fu, J.; Shou, J.-W.; Zhao, Z.-X.; Ren, L.; Wang, Y.; Jiang, J.-D. In Vitro Study of the Metabolic Characteristics of Eight Isoquinoline Alkaloids from Natural Plants in Rat Gut Microbiota. Molecules 2017, 22, 932.

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