Next Article in Journal
Five New Limonoids from Peels of Satsuma Orange (Citrus reticulata)
Previous Article in Journal
The Antidiabetic Mechanisms of Polyphenols Related to Increased Glucagon-Like Peptide-1 (GLP1) and Insulin Signaling
Article Menu
Issue 6 (June) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(6), 912; doi:10.3390/molecules22060912

Rapid Screening and Identification of Diterpenoids in Tinospora sinensis Based on High-Performance Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry

1
School of Chinese Pharmacy, Beijing University of Chinese Medicine, Beijing 100029, China
2
Beijing Research Institution of Chinese Medicine, Beijing University of Chinese Medicine, Beijing 100029, China
*
Correspondence: liubinyn67@163.com (B.L.)
*
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 20 February 2017 / Revised: 24 May 2017 / Accepted: 25 May 2017 / Published: 31 May 2017
View Full-Text   |   Download PDF [1299 KB, uploaded 2 June 2017]   |  

Abstract

Diterpenoids are considered the major active compounds in Tinospora sinensis in virtue of their special structures and activities. Herein, an analytical method was developed for rapid screening and identification of diterpenoids in T. sinensis using high-performmance liquid chromatography coupled with linear ion trap-Orbitrap mass spectrometry (HPLC-LTQ-Orbitrap) in negative ion mode. Two diterpenoid reference standards were first analyzed to obtain their characteristic ESI-MS/MS fragmentation patterns. Then, based on the extracted ion chromatogram (EIC) data-mining method and characteristic fragmentation pathways analysis, diterpenoids in T. sinensis were rapidly screened and identified. After that, an important parameter, Clog P, was adopted to discriminate between the isomers of diterpenoids. As a result, 63 diterpenoids were characterized from the extract of T. sinensis, including 10 diterpenoids and 53 diterpenoid glycosides. Among them, 15 compounds were tentatively identified as new compounds. Finally, target isolation of one diterpenoid glycoside named tinosineside A was performed based on the obtained results, which further confirmed the deduced fragmentation patterns and identified diterpenoid profile in T. sinensis. The results demonstrated that the established method could be a rapid, effective analytical tool for screening and characterization of diterpenoids in the complex systems of natural medicines. View Full-Text
Keywords: HPLC-LTQ-Orbitrap; diterpenoids; characteristic fragmentation pathways; Tinospora sinensis HPLC-LTQ-Orbitrap; diterpenoids; characteristic fragmentation pathways; Tinospora sinensis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Xu, L.-L.; Guo, F.-X.; Chi, S.-S.; Wang, Z.-J.; Jiang, Y.-Y.; Liu, B.; Zhang, J.-Y. Rapid Screening and Identification of Diterpenoids in Tinospora sinensis Based on High-Performance Liquid Chromatography Coupled with Linear Ion Trap-Orbitrap Mass Spectrometry. Molecules 2017, 22, 912.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top