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Molecules 2017, 22(5), 851; doi:10.3390/molecules22050851

Rapid and Cost-Effective Quantification of Glucosinolates and Total Phenolic Content in Rocket Leaves by Visible/Near-Infrared Spectroscopy

1
Department of Genomics and Biotecnology, IFAPA Center La Mojonera, Camino San Nicolás, La Mojonera 1, 04745 Almería, Spain
2
Department of Food and Health, IFAPA Center La Mojonera, Camino San Nicolás, La Mojonera 1, 04745 Almería, Spain
3
Department of Agronomy and Plant Breeding, Institute of Sustainable Agriculture, (CSIC), Alameda del Obispo s/n, 14080 Córdoba, Spain
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 27 April 2017 / Revised: 17 May 2017 / Accepted: 17 May 2017 / Published: 20 May 2017
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Abstract

The potential of visible-near infrared spectroscopy to predict glucosinolates and total phenolic content in rocket (Eruca vesicaria) leaves has been evaluated. Accessions of the E. vesicaria species were scanned by NIRS as ground leaf, and their reference values regressed against different spectral transformations by modified partial least squares (MPLS) regression. The coefficients of determination in the external validation (R2VAL) for the different quality components analyzed in rocket ranged from 0.59 to 0.84, which characterize those equations as having from good to excellent quantitative information. These results show that the total glucosinolates, glucosativin and glucoerucin equations obtained, can be used to identify those samples with low and high contents. The glucoraphanin equation obtained can be used for rough predictions of samples and in case of total phenolic content, the equation showed good correlation. The standard deviation (SD) to standard error of prediction ratio (RPD) and SD to range (RER) were variable for the different quality compounds and showed values that were characteristic of equations suitable for screening purposes or to perform accurate analyses. From the study of the MPLS loadings of the first three terms of the different equations, it can be concluded that some major cell components such as protein and cellulose, highly participated in modelling the equations for glucosinolates. View Full-Text
Keywords: glucosinolates; rocket; Eruca vesicaria; visible spectroscopy; near infrared; spectroscopy; chemometrics glucosinolates; rocket; Eruca vesicaria; visible spectroscopy; near infrared; spectroscopy; chemometrics
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Toledo-Martín, E.M.; Font, R.; Obregón-Cano, S.; De Haro-Bailón, A.; Villatoro-Pulido, M.; Del Río-Celestino, M. Rapid and Cost-Effective Quantification of Glucosinolates and Total Phenolic Content in Rocket Leaves by Visible/Near-Infrared Spectroscopy. Molecules 2017, 22, 851.

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