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Molecules 2017, 22(5), 777; doi:10.3390/molecules22050777

Crude Ecklonia cava Flake Extracts Attenuate Inflammation through the Regulation of TLR4 Signaling Pathway in LPS-Induced RAW264.7 Cells

Department of Food Science and Biotechnology, CHA University, Seongnam, Gyeonggi 463-400, Korea
These authors contributed equally to this work.
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Author to whom correspondence should be addressed.
Academic Editors: Dong-Kug Choi and Palanivel Ganesan
Received: 8 March 2017 / Revised: 5 April 2017 / Accepted: 28 April 2017 / Published: 10 May 2017
(This article belongs to the Special Issue Natural Products and Chronic Diseases)
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Abstract

We investigated the beneficial effects of the crude Ecklonia cava flake (CEF), which is a residual product after polyphenol extraction from Ecklonia cava, on inflammation in LPS-stimulated RAW264.7 cells. A group of five different CEF extracts was obtained by a preparation process using water, hydrochloric acid or temperature. We observed that large-size (>19 kDa) CEF extract, which was extracted with water at 95 °C (CEF-W, 95 °C), suppressed the production of inflammatory cytokines by inhibiting its mRNA expression in LPS-induced RAW264.7 cells. TLR4 signaling involvements were negatively regulated by CEF-W, 95 °C. CEF-W, 95 °C repressed the translocation of NF-κB from cytoplasm into nucleus in LPS-induced RAW264.7 cells. CEF-W, 95 °C attenuated the phosphorylation of TBK1 and IRF3 by inhibiting the phosphorylation of ERK. Taken together, we demonstrated that large-size CEF-W, 95 °C may act as a negative regulator of inflammation through the suppression of TLR4 signaling constituents in LPS-induced RAW264.7 cells. View Full-Text
Keywords: Ecklonia cava flake extract; inflammation; TLR4; NF-κB; IRF3; MAPKs Ecklonia cava flake extract; inflammation; TLR4; NF-κB; IRF3; MAPKs
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MDPI and ACS Style

Hwang, J.-H.; Kim, K.-J.; Lee, B.-Y. Crude Ecklonia cava Flake Extracts Attenuate Inflammation through the Regulation of TLR4 Signaling Pathway in LPS-Induced RAW264.7 Cells. Molecules 2017, 22, 777.

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