An Optimized Facile Procedure to Synthesize and Purify Allicin
AbstractAllicin is a reactive sulfur species (RSS) and defence substance from garlic (Allium sativum L.). The compound is a broad-spectrum antibiotic that is also effective against multiple drug resistant (MDR) strains. A detailed protocol for allicin synthesis based on diallyl-disulfide (DADS) oxidation by H2O2 using acetic acid as a catalyst was published in 2001 by Lawson and Wang. Here we report on improvements to this basic method, clarify the mechanism of the reaction and show that it is zero-order with respect to DADS and first-order with respect to the concentration of H2O2. The progress of allicin synthesis and the reaction mechanism were analyzsd by high-performance liquid chromatography (HPLC) and the identity and purity of the products was verified with LC-MS and 1H-NMR. We were able to obtain allicin of high purity (>98%) and >91% yield, with standard equipment available in any reasonable biological laboratory. This protocol will enable researchers to prepare and work with easily and cheaply prepared allicin of high quality. View Full-Text
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Albrecht, F.; Leontiev, R.; Jacob, C.; Slusarenko, A.J. An Optimized Facile Procedure to Synthesize and Purify Allicin. Molecules 2017, 22, 770.
Albrecht F, Leontiev R, Jacob C, Slusarenko AJ. An Optimized Facile Procedure to Synthesize and Purify Allicin. Molecules. 2017; 22(5):770.Chicago/Turabian Style
Albrecht, Frank; Leontiev, Roman; Jacob, Claus; Slusarenko, Alan J. 2017. "An Optimized Facile Procedure to Synthesize and Purify Allicin." Molecules 22, no. 5: 770.
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