Next Article in Journal
Synthesis of Gibberellic Acid Derivatives and Their Effects on Plant Growth
Previous Article in Journal
Relationship between Expression of Chalcone Synthase Genes and Chromones in Artificial Agarwood induced by Formic Acid Stimulation Combined with Fusarium sp. A2 Inoculation
Article Menu
Issue 5 (May) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(5), 681; doi:10.3390/molecules22050681

Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx

1
Agricultural college, Ningxia University, Yinchuan 750021, Ningxia, China
2
Innovative Drug Research Centre (IDRC), School of Pharmaceutical Sciences, Huxi Campus, Chongqing University, Chongqing 401331, China
*
Author to whom correspondence should be addressed.
Academic Editors: Thomas J. Schmidt and Bruno Figadere
Received: 15 February 2017 / Revised: 18 April 2017 / Accepted: 19 April 2017 / Published: 25 April 2017
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [574 KB, uploaded 25 April 2017]   |  

Abstract

Three indole derivatives, a novel benzoxazine-indole hybrid (1) and two known indole trimers (2, 3), were isolated from the metagenomic library of the marine sponge Discodermia calyx based on functional screening. Their structures were elucidated by extensive spectroscopic analysis and comparison of their NMR data to that of known compounds. The antibacterial assay indicated that only compound 2 displayed significant antibacterial activity against Bacillus cereus, with approximately 20 mm diameter growth inhibition at 10 µg/paper. HPLC analyses revealed that compound 2 is a newly induced metabolite, and the concentration of 3 was obviously enhanced in contrast to negative control, while 1 was not detected, allowing us to predict that the formation of 2 might be induced by exogenous genes derived from the sponge metagenome, whereas compound 1 could be formed through a non-enzymatic process during the isolation procedure. View Full-Text
Keywords: Discodermia calyx; indole derivative; antibacterial activity; metagenomics Discodermia calyx; indole derivative; antibacterial activity; metagenomics
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Liu, F.-L.; Yang, X.-L. Indole Derivatives Produced by the Metagenome Genes of the Escherichia coli-Harboring Marine Sponge Discodermia calyx. Molecules 2017, 22, 681.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top