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Molecules 2017, 22(4), 625; doi:10.3390/molecules22040625

Expression of Adenosine A2B Receptor and Adenosine Deaminase in Rabbit Gastric Mucosa ECL Cells

Department of Physiology, Faculty of Medicine and Nursing, University of the Basque Country UPV/EHU, Sarriena s/n, 48940 Leioa, Bizkaia, Spain
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Author to whom correspondence should be addressed.
Academic Editors: Francisco Ciruela and Eddy Sotelo
Received: 10 March 2017 / Revised: 10 April 2017 / Accepted: 11 April 2017 / Published: 12 April 2017
(This article belongs to the Special Issue Adenosine Receptors)
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Abstract

Adenosine is readily available to the glandular epithelium of the stomach. Formed continuously in intracellular and extracellular locations, it is notably produced from ATP released in enteric cotransmission. Adenosine analogs modulate chloride secretion in gastric glands and activate acid secretion in isolated parietal cells through A2B adenosine receptor (A2BR) binding. A functional link between surface A2BR and adenosine deaminase (ADA) was found in parietal cells, but whether this connection is a general feature of gastric mucosa cells is unknown. Here we examine whether A2BR is expressed at the membrane of histamine-producing enterochromaffin-like (ECL) cells, the major endocrine cell type in the oxyntic mucosa, and if so, whether it has a vicinity relationship with ADA. We used a highly homogeneous population of rabbit ECL cells (size 7.5–10 µm) after purification by elutriation centrifugation. The surface expression of A2BR and ADA proteins was assessed by flow cytometry and confocal microscopy. Our findings demonstrate that A2BR and ADA are partially coexpressed at the gastric ECL cell surface and that A2BR is functional, with regard to binding of adenosine analogs and adenylate cyclase activation. The physiological relevance of A2BR and ADA association in regulating histamine release is yet to be explained. View Full-Text
Keywords: adenosine; adenosine deaminase; G protein-coupled receptor; gastric neuroendocrine cell; enterochromaffin-like (ECL) cell adenosine; adenosine deaminase; G protein-coupled receptor; gastric neuroendocrine cell; enterochromaffin-like (ECL) cell
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MDPI and ACS Style

Arin, R.M.; Vallejo, A.I.; Rueda, Y.; Fresnedo, O.; Ochoa, B. Expression of Adenosine A2B Receptor and Adenosine Deaminase in Rabbit Gastric Mucosa ECL Cells. Molecules 2017, 22, 625.

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