The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen
AbstractWe describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. View Full-Text
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Sander, V.; Patke, S.; Lee, J.Y.; Chang, Y.-T.; Davidson, A.J. The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen. Molecules 2017, 22, 454.
Sander V, Patke S, Lee JY, Chang Y-T, Davidson AJ. The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen. Molecules. 2017; 22(3):454.Chicago/Turabian Style
Sander, Veronika; Patke, Shantanu; Lee, Jung Y.; Chang, Young-Tae; Davidson, Alan J. 2017. "The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen." Molecules 22, no. 3: 454.
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