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Molecules 2017, 22(3), 454; doi:10.3390/molecules22030454

The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen

1
Department of Molecular Medicine & Pathology, University of Auckland, Auckland 1142, New Zealand
2
Department of Chemistry & NUS MedChem Program of Life Sciences Institute, National University of Singapore and Singapore Bioimaging Consortium, A* STAR, Singapore 138667, Singapore
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 23 January 2017 / Revised: 9 March 2017 / Accepted: 11 March 2017 / Published: 13 March 2017
(This article belongs to the Section Molecular Diversity)
View Full-Text   |   Download PDF [5649 KB, uploaded 13 March 2017]   |  

Abstract

We describe the use of the fluorescent reporter compound CDr10b to label mid-intestinal structures in zebrafish larvae after simple immersion. CDr10b is deposited into the gut where it initially fills the lumen and is excreted. Using laser-mediated injury of the intestine, we show that CDr10b provides a useful readout of the integrity and repair of the epithelial cell barrier. In addition, CDr10b specifically labels the absorptive mid-intestine segment that is analogous to the mammalian small intestine. By perturbing retinoic acid signaling, which regulates the size of the mid-intestine segment, we show that CDr10b is a valuable tool to rapidly assess developmental malformations of the intestine in live animals. View Full-Text
Keywords: zebrafish; gut development; mid-intestine; CDr10b; vital dye; laser ablation zebrafish; gut development; mid-intestine; CDr10b; vital dye; laser ablation
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sander, V.; Patke, S.; Lee, J.Y.; Chang, Y.-T.; Davidson, A.J. The Vital Dye CDr10b Labels the Zebrafish Mid-Intestine and Lumen. Molecules 2017, 22, 454.

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