Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca2+/CaMKII/CREB Signaling Pathway
Abstract
:1. Introduction
2. Results
2.1. Effect of PF on Neurological Deficit Scores and the Infarct Volume of Rats
2.2. Effect of PF on the Expression of Proteins in the Ca2+/CaMKII/CREB Signaling Pathway in Ischemic Penumbra after MCAO
2.3. Effect of PF on Cell Viability in Primary Hippocampal Neurons
2.4. Effect of PF on Cell Apoptosis in Primary Hippocampal Neurons
2.5. Effect of PF on Intracellular Ca2+ Concentration
2.6. Effect of PF on the Expression of Proteins in the Ca2+/CaMKII/CREB Signaling Pathway in Primary Hippocampal Neurons after NMDA-Induced Excitotoxicity
3. Discussion
4. Materials and Methods
4.1. Reagents and Animals
4.2. Middle Cerebral Artery Occlusion and Reperfusion (MCAO) Model
4.3. Experimental Groups and Treatment
4.4. Evaluation of Neurological Deficit
4.5. Measurement of Ischemic Infarct Area
4.6. Primary Hippocampal Neuron Culture and NMDA-Induced Excitotoxicity Model
4.7. Cell Viability Assay
4.8. Flow Cytometric Analysis
4.9. Intracellular Ca2+ Measurement
4.10. Western Blot Analysis
4.11. Statistical Analysis
5. Conclusions
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Zhang, Y.; Qiao, L.; Xu, W.; Wang, X.; Li, H.; Xu, W.; Chu, K.; Lin, Y. Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca2+/CaMKII/CREB Signaling Pathway. Molecules 2017, 22, 359. https://doi.org/10.3390/molecules22030359
Zhang Y, Qiao L, Xu W, Wang X, Li H, Xu W, Chu K, Lin Y. Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca2+/CaMKII/CREB Signaling Pathway. Molecules. 2017; 22(3):359. https://doi.org/10.3390/molecules22030359
Chicago/Turabian StyleZhang, Yuqin, Lifei Qiao, Wen Xu, Xiaoying Wang, Huang Li, Wei Xu, Kedan Chu, and Yu Lin. 2017. "Paeoniflorin Attenuates Cerebral Ischemia-Induced Injury by Regulating Ca2+/CaMKII/CREB Signaling Pathway" Molecules 22, no. 3: 359. https://doi.org/10.3390/molecules22030359