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Molecules 2017, 22(2), 271; doi:10.3390/molecules22020271

A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice

1
Department of Biochemistry and Molecular Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil
2
Department of Biology, Federal University of Ceara, Fortaleza 60440-900, Brazil
3
Department of Molecular Biology, Federal University of Paraiba, Joao Pessoa 58051-900, Brazil
4
Department of Physiology and Pharmacology, Federal University of Ceara, Fortaleza 60430-160, Brazil
*
Authors to whom correspondence should be addressed.
Academic Editor: Philippe Bulet
Received: 17 January 2017 / Revised: 7 February 2017 / Accepted: 9 February 2017 / Published: 11 February 2017
(This article belongs to the Special Issue Bioactive Natural Peptides As A Pipeline For Therapeutics)
View Full-Text   |   Download PDF [4220 KB, uploaded 11 February 2017]   |  

Abstract

Moringa oleifera has been used in traditional medicine to treat diabetes. However, few studies have been conducted to relate its antidiabetic properties to proteins. In this study, a leaf protein isolate was obtained from M. oleifera leaves, named Mo-LPI, and the hypoglycemic and antioxidant effects on alloxan-induced diabetic mice were assessed. Mo-LPI was obtained by aqueous extraction, ammonium sulphate precipitation and dialysis. The electrophoresis profile and proteolytic hydrolysis confirmed its protein nature. Mo-LPI showed hemagglutinating activity, cross-reaction with anti-insulin antibodies and precipitation after zinc addition. Single-dose intraperitoneal (i.p.) administration of Mo-LPI (500 mg/kg·bw) reduced the blood glucose level (reductions of 34.3%, 60.9% and 66.4% after 1, 3 and 5 h, respectively). The effect of Mo-LPI was also evidenced in the repeated dose test with a 56.2% reduction in the blood glucose level on the 7th day after i.p. administration. Mo-LPI did not stimulate insulin secretion in diabetic mice. Mo-LPI was also effective in reducing the oxidative stress in diabetic mice by a decrease in malondialdehyde level and increase in catalase activity. Mo-LPI (2500 mg/kg·bw) did not cause acute toxicity to mice. Mo-LPI is a promising alternative or complementary agent to treat diabetes. View Full-Text
Keywords: Moringa; plant protein; hypoglycemic activity; antioxidant activity; diabetes therapy Moringa; plant protein; hypoglycemic activity; antioxidant activity; diabetes therapy
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MDPI and ACS Style

Paula, P.C.; Sousa, D.O.B.; Oliveira, J.T.A.; Carvalho, A.F.U.; Alves, B.G.T.; Pereira, M.L.; Farias, D.F.; Viana, M.P.; Santos, F.A.; Morais, T.C.; Vasconcelos, I.M. A Protein Isolate from Moringa oleifera Leaves Has Hypoglycemic and Antioxidant Effects in Alloxan-Induced Diabetic Mice. Molecules 2017, 22, 271.

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