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Molecules 2017, 22(11), 1893; doi:10.3390/molecules22111893

Structural and Functional Properties Changes of β-Conglycinin Exposed to Hydroxyl Radical-Generating Systems

1
College of Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China, xujing@neau.edu.cn (J.X.)
2
College of Food Science, Northeast Agricultural University, Harbin 150030, Heilongjiang, China
*
Authors to whom correspondence should be addressed.
Received: 9 October 2017 / Accepted: 1 November 2017 / Published: 3 November 2017
(This article belongs to the Collection Bioactive Compounds)
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Abstract

The objective of the present study was to examine the structural and functional changes of β-conglycinin exposed to oxidizing radicals produced by FeCl3/H2O2/ascorbic acid hydroxyl radical-generating system (HRGS) for 3 h at room temperature. Increasing H2O2 concentrations resulted in a loss of histidine residues, lysine residues, and available lysine, which was accompanied by the formation of protein carbonyls and disulphide bonds (p < 0.05). Changes in secondary structure, surface hydrophobicity, and intrinsic fluorescence indicated that hydroxyl radicals had induced protein unfolding and conformational alterations. Results from SDS-PAGE implied that a small amount of protein cross-linkages produced by oxidative incubation. The emulsifying properties of β-conglycinin were gradually improved with the increasing extent of oxidation. The structural changes above contributed to the reduction of potential allergenicity of β-conglycinin, as verified by specific ELISA analysis. These results suggest that moderate oxidation could partially improve the protein functional properties and reduced the potential allergy of protein, providing guidance for effective use of moderately oxidized soy protein in the industry. View Full-Text
Keywords: β-conglycinin; hydroxyl-radical; protein oxidation; emulsifying properties; allergenicity β-conglycinin; hydroxyl-radical; protein oxidation; emulsifying properties; allergenicity
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MDPI and ACS Style

Xu, J.; Chen, Z.; Han, D.; Li, Y.; Sun, X.; Wang, Z.; Jin, H. Structural and Functional Properties Changes of β-Conglycinin Exposed to Hydroxyl Radical-Generating Systems. Molecules 2017, 22, 1893.

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