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Molecules 2017, 22(11), 1823; doi:10.3390/molecules22111823

Asperflavin, an Anti-Inflammatory Compound Produced by a Marine-Derived Fungus, Eurotium amstelodami

1
College of Chemical and Pharmaceutical Engineering, Jilin Institute of Chemical Technology, Jilin 132022, China
2
Department of Marine Life Science, Jeju National University, Jeju 690-756, Korea
3
College of Pharmacy Science, Changchun University of Traditional Chinese Medicine, Changchun 130017, China
4
Pediatric Oncology Experimental Therapeutics Investigators Consortium (POETIC) Laboratory for Pre-Clinical and Drug Discovery Studies, University of Calgary, Calgary, AB T2N1N4, Canada
5
Marine and Environmental Research Institute, Jeju National University, Jeju 695-814, Korea
*
Author to whom correspondence should be addressed.
Received: 21 September 2017 / Revised: 15 October 2017 / Accepted: 24 October 2017 / Published: 29 October 2017
(This article belongs to the Special Issue Biological Activity of Secondary Metabolites)
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Abstract

In the present study, 16 marine-derived fungi were isolated from four types of marine materials including float, algae, animals and drift woods along with the coast of Jeju Island, Korea and evaluated for anti-inflammatory effects in lipopolysaccharide (LPS)-stimulated RAW 24.7 cells. The broth and mycelium extracts from the 16 fungi were prepared and the broth extract (BE) of Eurotium amstelodami (015-2) inhibited nitric oxide (NO) production in LPS-stimulated RAW 264.7 cells without cytotoxicity. By further bioassay-guided isolation, three compounds including asperflavin, neoechinulin A and preechinulin were successfully isolated from the BE of E. amstelodami. It was revealed that asperflavin showed no cytotoxicity up to 200 μM and significantly inhibited LPS-induced NO and PGE2 production in a dose-dependent manner. In the western blot results, asperflavin suppressed only inducible NOS (iNOS), but COX-2 were slightly down-regulated. Asperflavin was also observed to inhibit the production of pro-inflammatory cytokines including TNF-α, IL-1β, and IL-6. In conclusion, this study reports a potential use of asperflavin isolated from a marine fungus, E. amstelodami as an anti-inflammatory agent via suppression of iNOS and pro-inflammatory cytokines as well as no cytotoxicity. View Full-Text
Keywords: asperflavin; marine fungus; Anti-inflammation; Eurotium amstelodami; NO production asperflavin; marine fungus; Anti-inflammation; Eurotium amstelodami; NO production
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Yang, X.; Kang, M.-C.; Li, Y.; Kim, E.-A.; Kang, S.-M.; Jeon, Y.-J. Asperflavin, an Anti-Inflammatory Compound Produced by a Marine-Derived Fungus, Eurotium amstelodami. Molecules 2017, 22, 1823.

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