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Molecules 2017, 22(12), 2089; doi:10.3390/molecules22122089

HPLC-PDA-MS/MS Characterization of Bioactive Secondary Metabolites from Turraea fischeri Bark Extract and Its Antioxidant and Hepatoprotective Activities In Vivo

1
Institute of Pharmacy and Molecular Biotechnology, Heidelberg University, Im Neuenheimer Feld 364, Heidelberg 69120, Germany
2
Department of Pharmacology and Toxicology, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
3
Department of Pharmacognosy, College of Pharmacy, Cairo University, Cairo 11562, Egypt
4
Department of Pharmacognosy, Faculty of Pharmacy, Zagazig University, Zagazig 44519, Egypt
*
Authors to whom correspondence should be addressed.
Received: 6 November 2017 / Revised: 21 November 2017 / Accepted: 27 November 2017 / Published: 29 November 2017
(This article belongs to the Special Issue Biological Activity of Secondary Metabolites)
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Abstract

Turraea fischeri is an East African traditional herb, which is widely used in traditional medicine. In this study, we profiled the secondary metabolites in the methanol extract of T. fischeri bark using HPLC-PDA-ESI-MS/MS, and 20 compounds were tentatively identified. Several isomers of the flavonolignan cinchonain-I and bis-dihydroxyphenylpropanoid-substituted catechin hexosides dominated the extract. Robust in vitro and in vivo antioxidant properties were observed in 1,1-diphenyl-2-picrylhydrazyl radical scavenging assay (DPPH) and ferric reducing antioxidant power (FRAP) assay, and in the model organism Caenorhabditis elegans. Additionally, the extract exhibited promising hepatoprotective activities in D-galactosamine (D-GaIN) treated rats. A significant reduction in the elevated levels of aspartate aminotransferase (AST), total bilirubin, gamma-glutamyltransferase (GGT), and malondialdehyde (MDA) and increase of glutathione (GSH) was observed in rats treated with the bark extract in addition to D-galactosamine when compared with rats treated with D-galactosamine alone. In conclusion, T. fischeri is apromising candidate for health-promoting and for pharmaceutical applications. View Full-Text
Keywords: Turraea fischeri; flavonolignan; cinchonains; HPLC-PDA-ESI-MS/MS; antioxidant; hepatoprotection Turraea fischeri; flavonolignan; cinchonains; HPLC-PDA-ESI-MS/MS; antioxidant; hepatoprotection
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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MDPI and ACS Style

Sobeh, M.; Mahmoud, M.F.; Sabry, O.M.; Adel, R.; Dmirieh, M.; El-Shazly, A.M.; Wink, M. HPLC-PDA-MS/MS Characterization of Bioactive Secondary Metabolites from Turraea fischeri Bark Extract and Its Antioxidant and Hepatoprotective Activities In Vivo. Molecules 2017, 22, 2089.

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