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Molecules 2017, 22(10), 1694; doi:10.3390/molecules22101694

PrLPAAT4, a Putative Lysophosphatidic Acid Acyltransferase from Paeonia rockii, Plays an Important Role in Seed Fatty Acid Biosynthesis

1
College of Landscape Architecture and Arts, Northwest A&F University, Yangling, Shaanxi 712100, China
2
Institute of Biochemistry and Cell Biology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200031, China
These authors contributed equally to this work.
*
Authors to whom correspondence should be addressed.
Received: 14 September 2017 / Accepted: 3 October 2017 / Published: 10 October 2017
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Abstract

Lysophosphatidic acid acyltransferases (LPAATs) are essential for the acylation of lysophosphatidic acid (LPA) and the synthesis of phosphatidic acid (PA), a key intermediate in the synthesis of membrane phospholipids and storage lipids. Here, a putative lysophosphatidic acid acyltransferase gene, designated PrLPAAT4, was isolated from seed unsaturated fatty acid (UFA)-rich P. rockii. The complete PrLPAAT4 cDNA contained a 1116-bp open reading frame (ORF), encoding a 42.9 kDa protein with 371 amino acid residues. Bioinformatic analysis indicates that PrLPAAT4 is a plasma membrane protein belonging to acyl-CoA:1-acylglycerol-sn-3-phosphate acyltranferases (AGPAT) family. PrLPAAT4 shared high sequence similarity with its homologs from Citrus clementina, Populus trichocarpa, Manihot esculenta, and Ricinus communis. In Arabidopsis, overexpression of PrLPAAT4 resulted in a significant increase in the content of oleic acid (OA) and total fatty acids (FAs) in seeds. AtDGAT1, AtGPAT9, and AtOleosin, involved in TAG assembly, were upregulated in PrLPAAT4-overexpressing lines. These results indicated that PrLPAAT4 functions may be as a positive regulator in seed FA biosynthesis. View Full-Text
Keywords: lysophosphatidic acid acyltransferase; triacylglycerol; tree peony; Arabidopsis; oleic acid; fatty acid lysophosphatidic acid acyltransferase; triacylglycerol; tree peony; Arabidopsis; oleic acid; fatty acid
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Zhang, Q.; Yu, R.; Sun, D.; Bai, Z.; Li, H.; Xue, L.; Zhang, Y.; Niu, L. PrLPAAT4, a Putative Lysophosphatidic Acid Acyltransferase from Paeonia rockii, Plays an Important Role in Seed Fatty Acid Biosynthesis. Molecules 2017, 22, 1694.

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