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Molecules 2017, 22(10), 1659; doi:10.3390/molecules22101659

Effect of High-Pressure Treatment on Catalytic and Physicochemical Properties of Pepsin

School of Food Science, Henan Institute of Science and Technology, Xinxiang 453003, China
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Received: 19 September 2017 / Revised: 29 September 2017 / Accepted: 2 October 2017 / Published: 11 October 2017
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Abstract

For a long time, high-pressure treatment has been used to destroy the compact structures of natural proteins in order to promote subsequent enzymatic hydrolysis. However, there are few reports evaluating the feasibility of directly improving the catalytic capability of proteases by using high-pressure treatments. In this study, the effects of high-pressure treatment on the catalytic capacity and structure of pepsin were investigated, and the relationship between its catalytic properties and changes in its physicochemical properties was explored. It was found that high-pressure treatment could lead to changes of the sulfhydryl group/disulfide bond content, hydrophobicity, hydrodynamic radius, intrinsic viscosity, and subunit composition of pepsin, and the conformational change of pepsin resulted in improvement to its enzymatic activity and hydrolysis efficiency, which had an obvious relationship with the high-pressure treatment conditions. View Full-Text
Keywords: high-pressure treatment; pepsin; enzymatic hydrolysis; physicochemical properties high-pressure treatment; pepsin; enzymatic hydrolysis; physicochemical properties
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Wang, J.; Bai, T.; Ma, Y.; Ma, H. Effect of High-Pressure Treatment on Catalytic and Physicochemical Properties of Pepsin. Molecules 2017, 22, 1659.

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