Next Article in Journal
Atom-Economic Synthesis of 4-Pyrones from Diynones and Water
Previous Article in Journal
Stepwise, Protecting Group Free Synthesis of [4]Rotaxanes
Article Menu
Issue 1 (January) cover image

Export Article

Open AccessArticle
Molecules 2017, 22(1), 96; doi:10.3390/molecules22010096

Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes

1
School of Basic Medical Science, Ningxia Medical University, Yinchuan 750004, China
2
School of Pharmacy, Ningxia Medical University, Yinchuan 750004, China
3
China National Center for Biotechnology Development, Beijing 100039, China
4
School of Chinese Medicine, Ningxia Medical University, Yinchuan 750004, China
5
Key Laboratory of Hui Medicine Modernization, Ministry of Education, Yinchuan 750004, China
6
Tongji Hospital, Huazhong University of Science and Technology, Wuhan 430030, China
7
Ningxia Engineering and Technology Research Center for Modernization of Hui Medicine, Yinchuan 750004, China
These authors contributed equally to this work.
*
Author to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 8 November 2016 / Revised: 1 January 2017 / Accepted: 4 January 2017 / Published: 10 January 2017
(This article belongs to the Section Natural Products)
View Full-Text   |   Download PDF [1403 KB, uploaded 10 January 2017]   |  

Abstract

Chemical investigation of Tamarix ramosissima Ledeb, a traditional herbal medicine used for rheumatoid arthritis (RA) treatment in northwest China, led to the discovery of a new phenolic aromatic rings substituted lactam, tamaractam (1), together with the previously reported compounds cis-N-feruloyl-3-O-methyldopamine (2) and trans-N-feruloyl-3-O-methyldopamine (3). The structures of the compounds were determined by high resolution electrospray ionization mass spectroscopy (HRESIMS) and 1D and 2D-NMR experiments, as well as comparison with the literature data. The effects of the three compounds on the viability of RA fibroblast-like synoviocytes (RA-FLS) were assessed by 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2-H-tetrazolium bromide (MTT) assay. Pro-apoptosis effect of compound 1 in RA-FLS was further investigated by terminal deoxynucleotidyl transferase-mediated dUTP nick-end labeling (TUNEL) assay, activated caspase-3/7 level assessment using luminescence assay, and sub-G1 fraction measurement using flow cytometry. It was found that these three compounds displayed variable proliferation inhibitory activity in RA-FLS, and compound 1 exhibited the strongest effect. Compound 1 could remarkably induce cellular apoptosis of RA-FLS, increase activated caspase-3/7 levels, and significantly increase sub-G1 fraction in the cell cycle. The results suggested that compound 1 may inhibit the proliferation of RA-FLS through apoptosis-inducing effect, and these compounds may contribute to the anti-RA effect of T. ramosissima. View Full-Text
Keywords: Tamarix ramosissima; tamaractam; rheumatoid arthritis; fibroblast-like synoviocytes; growth inhibitory activity; apoptosis Tamarix ramosissima; tamaractam; rheumatoid arthritis; fibroblast-like synoviocytes; growth inhibitory activity; apoptosis
Figures

Figure 1

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

Supplementary material

Scifeed alert for new publications

Never miss any articles matching your research from any publisher
  • Get alerts for new papers matching your research
  • Find out the new papers from selected authors
  • Updated daily for 49'000+ journals and 6000+ publishers
  • Define your Scifeed now

SciFeed Share & Cite This Article

MDPI and ACS Style

Yao, Y.; Jiang, C.-S.; Sun, N.; Li, W.-Q.; Niu, Y.; Han, H.-Q.; Miao, Z.-H.; Zhao, X.-X.; Zhao, J.; Li, J. Tamaractam, a New Bioactive Lactam from Tamarix ramosissima, Induces Apoptosis in Rheumatoid Arthritis Fibroblast-Like Synoviocytes. Molecules 2017, 22, 96.

Show more citation formats Show less citations formats

Note that from the first issue of 2016, MDPI journals use article numbers instead of page numbers. See further details here.

Related Articles

Article Metrics

Article Access Statistics

1

Comments

[Return to top]

Molecules EISSN 1420-3049 Published by MDPI AG, Basel, Switzerland RSS E-Mail Table of Contents Alert
Back to Top