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Molecules 2017, 22(1), 186; doi:10.3390/molecules22010186

Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents

1
Institute of Molecular Science, Key Laboratory of Chemical Biology and Molecular Engineering of Ministry of Education, Shanxi University, Taiyuan 030006, China
2
College of Chemistry and Chemical Engineering, Shanxi University, Taiyuan 030006, China
*
Author to whom correspondence should be addressed.
Academic Editor: Hua Zhao
Received: 31 October 2016 / Revised: 18 January 2017 / Accepted: 19 January 2017 / Published: 22 January 2017
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Abstract

Daidzein, which is scarce in nature, has gained significant attention due to its superior biological activity and bioavailability compared with daidzin. So far, it has been widely used in the medicine and health care products industries. The enzymatic approach for the preparation of daidzein has prevailed, benefitted by its high efficiency and eco-friendly nature. Our present research aimed at providing a preparation method of daidzein by enzymatic hydrolysis of daidzin in a new “green” reaction medium-deep eutectic solvents (DESs). Herein, the DESs were screened via evaluating enzyme activity, enzyme stability and the substrate solubility, and the DES (ChCl/EG 2:1, 30 vol %) was believed to be the most appropriate co-solvent to improve the bioconversion efficiency. Based on the yield of daidzein, response surface methodology (RSM) was employed to model and optimize the reaction parameters. Under these optimum process conditions, the maximum yield of 97.53% was achieved and the purity of daidzein crude product reached more than 70%, which is more efficient than conversions in DESs-free buffer. Importantly, it has been shown that DESs medium could be reused for six batches of the process with a final conversion of above 50%. The results indicated that this procedure could be considered a mild, environmentally friendly, highly efficient approach to the economical production of daidzein, with a simple operation process and without any harmful reagents being involved. View Full-Text
Keywords: deep eutectic solvents; daidzein; enzymatic hydrolysis; response surface methodology; optimization deep eutectic solvents; daidzein; enzymatic hydrolysis; response surface methodology; optimization
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Cheng, Q.-B.; Zhang, L.-W. Highly Efficient Enzymatic Preparation of Daidzein in Deep Eutectic Solvents. Molecules 2017, 22, 186.

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