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Molecules 2017, 22(1), 17; doi:10.3390/molecules22010017

Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine

1
Institut für Experimentalphysik, Freie Universität Berlin, Arnimallee 14, 14195 Berlin, Germany
2
Institut für Chemie und Biochemie, Freie Universität Berlin, Takustrasse 3, 14195 Berlin, Germany
3
Helmholtz Virtual Institute—Multifunctional Biomaterials for Medicine, Helmholtz-Zentrum Geesthacht, Kantstr. 55, 14513 Teltow, Germany
*
Authors to whom correspondence should be addressed.
Academic Editor: Didier Astruc
Received: 16 September 2016 / Revised: 16 December 2016 / Accepted: 16 December 2016 / Published: 24 December 2016
(This article belongs to the Collection Nanomedicine)
View Full-Text   |   Download PDF [6036 KB, uploaded 24 December 2016]   |  

Abstract

The emerging field of nanomedicine provides new approaches for the diagnosis and treatment of diseases, for symptom relief and for monitoring of disease progression. One route of realizing this approach is through carefully constructed nanoparticles. Due to the small size inherent to the nanoparticles a proper characterization is not trivial. This review highlights the application of time-resolved fluorescence spectroscopy and fluorescence lifetime imaging microscopy (FLIM) for the analysis of nanoparticles, covering aspects ranging from molecular properties to particle detection in tissue samples. The latter technique is particularly important as FLIM allows for distinguishing of target molecules from the autofluorescent background and, due to the environmental sensitivity of the fluorescence lifetime, also offers insights into the local environment of the nanoparticle or its interactions with other biomolecules. Thus, these techniques offer highly suitable tools in the fields of particle development, such as organic chemistry, and in the fields of particle application, such as in experimental dermatology or pharmaceutical research. View Full-Text
Keywords: nanomedicine; dendritic nanoparticles; time-resolved fluorescence depolarization; fluorescence lifetime imaging microscopy; FLIM nanomedicine; dendritic nanoparticles; time-resolved fluorescence depolarization; fluorescence lifetime imaging microscopy; FLIM
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This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Boreham, A.; Brodwolf, R.; Walker, K.; Haag, R.; Alexiev, U. Time-Resolved Fluorescence Spectroscopy and Fluorescence Lifetime Imaging Microscopy for Characterization of Dendritic Polymer Nanoparticles and Applications in Nanomedicine. Molecules 2017, 22, 17.

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