HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus
Abstract
:1. Introduction
2. Results
2.1. Qualitative Analysis of Leaf Flavonoids of O. fragrans
2.2. Component Analysis of Leaf Samples of O. fragrans
2.3. Cluster Analysis of Different Cultivars
3. Discussion
4. Materials and Methods
4.1. Plant Materials
4.2. Chemicals and Reagents
4.3. Extraction of Flavonoids
4.4. Quantitative Determination of Flavonoids
4.5. Identification of Flavonoids
4.6. Statistical Analysis
Acknowledgments
Author Contributions
Conflicts of Interest
References
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Peaks a | tR (min) | λmax (nm) | ESI-PI MS/MS2 (m/z) | Tentative Identification |
---|---|---|---|---|
1 | 16.26 | 272, 326 | 611.1[M + H]+, 427.1, 303[Y0+] | Quercetin 7-O-neohesperidoside |
2 | 17.93 | 270, 334 | 595.1[M + H]+, 457.1, 385.1, 317.0[Y0+] | Isorhamnetin derivative |
3 | 20.78 | 238, 325 | 564.3[M + H]+, 410.1 | No tentative identification |
4 | 20.99 | 289, 332 | 449.1[M + H]+, 265.0, 211.0 | No tentative identification |
5 | 24.02 | 252, 334 | 611.1[M + H]+, 465.0, 303[Y0+] | Quercetin 3-O-rutinose |
6 | 24.66 | 253, 345 | 595.1[M + H]+, 448.8 (82) [Y1+], 433 (4), 287.1[Y0+] (100) | Luteolin 7-O-neohesperidoside |
7 | 25.43 | 266, 345 | 448.8[M + H]+, 287.1[Y0+] | Kaempferol 7-O-glucoside |
8 | 25.80 | 284, 330 | 449.1[M + H]+, 303[Y0+] | Quercitrin 3-O-rhamnoside |
9 | 25.94 | 281, 329 | 465.0[M + H]+, 303.1[Y0+] | Quercitrin 3-O-glucoside |
10 | 26.68 | 265, 332 | 341.1[M + H]+, 209.0[Y0+] | Esculin |
11 | 27.69 | 266, 334 | 578.9[M + H]+, 432.8(65) [Y1+], 271.1[Y0+](100) | Apigenin 7-O-neohesperidoside |
12 | 28.23 | 284, 332 | 435.2[M + H]+, 273.2[Y0+] | Naringenin 7-O-glucoside |
13 | 29.04 | 267, 333 | 432.8[M + H]+, 271.1[Y0+] | Apigenin 7-O-glucoside |
14 | 29.46 | 268, 335 | 448.9[M + H]+, 287.1[Y0+] | Kaempferol 3-O-glucoside |
15 | 29.74 | 268, 324 | 435.1[M + H]+, 322.1, 209.0[Y0+] | Fraxetin derivative |
Sample a No. | Contents (mg/g, DW) | |||||||||||||||
---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|---|
1 b | 2 | 3 | 4 | 5 | 6 | 7 | 8 | 9 | 10 | 11 | 12 | 13 | 14 | 15 | TF | |
C1 | 0.59 | 6.11 | 1.03 | 0.33 | 9.04 | 1.96 | 3.25 | 0.84 | 0.77 | 1.03 | 4.06 | 0.67 | 2.33 | 5.73 | 2.01 | 39.75 |
C2 | 0.71 | 4.25 | 0.63 | 0.36 | 7.94 | 2.37 | 2.86 | 0.45 | 0.76 | 0.82 | 3.25 | 0.58 | 0.91 | 6.40 | 1.24 | 33.52 |
C3 | 0.55 | 5.02 | 0.51 | 0.26 | 8.02 | 1.43 | 3.30 | 0.94 | 0.77 | 0.81 | 2.98 | 0.70 | 1.78 | 4.53 | 1.35 | 32.93 |
C4 | 0.49 | 4.20 | 0.98 | 0.23 | 9.54 | 0.40 | 1.42 | 0.46 | 1.85 | 0.84 | 2.87 | 0.52 | 1.17 | 1.79 | 1.16 | 27.91 |
C5 | 0.35 | 2.29 | 0.46 | 0.19 | 14.86 | 1.06 | 2.75 | 1.10 | 1.96 | 0.55 | 1.93 | 0.81 | 2.42 | 4.18 | 1.45 | 36.01 |
C6 | 0.85 | 4.11 | 0.76 | 0.39 | 9.70 | 1.32 | 2.99 | 0.75 | 0.92 | 0.63 | 1.89 | 0.46 | 1.09 | 5.70 | 0.99 | 32.54 |
C7 | 0.39 | 2.04 | 0.32 | 0.22 | 12.27 | 0.97 | 2.35 | 1.30 | 0.82 | 0.68 | 2.23 | 0.72 | 1.34 | 4.38 | 1.17 | 31.19 |
C8 | 0.38 | 2.25 | 0.73 | 0.17 | 9.67 | 1.38 | 3.96 | 1.07 | 0.74 | 1.12 | 1.77 | 0.52 | 2.14 | 5.34 | 0.95 | 32.18 |
C9 | 0.36 | 2.49 | 0.35 | 0.19 | 9.53 | 1.14 | 2.98 | 0.75 | 1.51 | 0.85 | 2.13 | 0.81 | 1.81 | 5.05 | 1.44 | 30.89 |
C10 | 0.33 | 2.44 | 0.59 | 0.16 | 4.41 | 1.18 | 3.88 | n.d. | 0.42 | 0.77 | 1.50 | 0.53 | 1.77 | 2.15 | 0.48 | 20.60 |
C11 | 0.77 | 2.67 | 0.53 | 0.33 | 14.74 | 1.48 | 4.56 | 0.88 | 1.54 | 1.63 | 2.24 | 1.00 | 2.00 | 3.85 | 0.94 | 39.15 |
C12 | 0.58 | 3.26 | 0.67 | 0.23 | 8.09 | 3.16 | 3.55 | 1.01 | 0.77 | 0.73 | 3.37 | 0.73 | 1.70 | 2.72 | 0.56 | 31.12 |
C13 | 0.20 | 1.97 | 0.72 | 0.18 | 5.85 | 1.54 | 2.23 | 0.88 | 0.58 | 0.51 | 3.25 | 0.67 | 1.53 | 3.18 | 0.88 | 24.18 |
C14 | 0.10 | 1.79 | 0.65 | 0.09 | 5.25 | 1.02 | 1.77 | 0.79 | 0.64 | 0.27 | 3.25 | 0.41 | 1.35 | 1.56 | 0.87 | 19.80 |
C15 | 0.54 | 2.32 | 0.66 | 0.36 | 11.22 | 1.25 | 2.74 | 0.72 | 1.13 | 1.19 | 1.73 | 0.53 | 0.99 | 4.52 | 0.97 | 30.85 |
C16 | 0.68 | 2.74 | 0.29 | 0.31 | 7.16 | 2.09 | 2.48 | 0.53 | 0.52 | 0.56 | 2.35 | 0.82 | 0.63 | 1.52 | 0.39 | 23.05 |
C17 | 0.59 | 2.09 | 0.43 | 0.35 | 16.33 | 2.20 | 3.87 | 1.10 | 1.97 | 0.91 | 2.80 | 1.22 | 0.82 | 6.44 | 0.82 | 41.57 |
C18 | 0.60 | 2.17 | 1.33 | 0.44 | 16.06 | 1.48 | 3.28 | n.d. | 2.09 | 0.53 | 2.32 | 0.75 | 0.97 | 5.33 | 0.67 | 38.01 |
C19 | 0.93 | 4.12 | 0.68 | 0.53 | 2.08 | 2.53 | 7.34 | n.d. | 0.62 | 0.63 | 3.49 | 0.71 | 5.67 | 9.00 | 1.35 | 39.72 |
C20 | 0.45 | 4.80 | 0.42 | 0.30 | 5.80 | 1.63 | 2.33 | n.d. | 0.91 | 0.88 | 3.46 | 0.96 | 1.04 | 3.94 | 0.98 | 27.88 |
C21 | 0.66 | 4.03 | 0.80 | 0.50 | 12.66 | 3.27 | 1.69 | 0.97 | 1.48 | 1.31 | 4.43 | 0.93 | 0.56 | 5.24 | 0.87 | 39.38 |
C22 | 0.91 | 2.74 | 0.36 | 0.56 | 12.19 | 2.48 | 3.71 | n.d. | 1.17 | 0.61 | 3.69 | 0.86 | 0.80 | 4.76 | 0.78 | 35.62 |
Cultivar Groups | Sample No. | Cultivars |
---|---|---|
Albus Group | C1 | O. fragrans ‘Xiaoye Sugui’ |
C2 | O. fragrans ‘Zaoyin Gui’ | |
C3 | O. fragrans ‘Yu Linglong’ | |
C4 | O. fragrans ‘Ziyin Gui’ | |
Luteus Group | C5 | O. fragrans ‘Boye Jingui’ |
C6 | O. fragrans ‘Hangzhou Huang’ | |
C7 | O. fragrans ‘Jin Qiu’ | |
Aurantiacus Group | C8 | O. fragrans ‘Ruanye Dangui’ |
C9 | O. fragrans ‘Chilian Jindan’ | |
C10 | O. fragrans ‘Mantiao Hong’ | |
C11 | O. fragrans ‘Wuyi Dangui’ | |
C12 | O. fragrans ‘Xionghuang’ | |
C13 | O. fragrans ‘Zhuangyuan Hong’ | |
C14 | O. fragrans ‘Chenghong Dangui’ | |
C15 | O. fragrans ‘Zhusha Dangui’ | |
C16 | O. fragrans ‘Yan Hong’ | |
C17 | O. fragrans ‘Yingye Dangui’ | |
Asiaticus Group | C18 | O. fragrans ‘Danzhuang’ |
C19 | O. fragrans ‘Sijigui’ | |
C20 | O. fragrans ‘Tiannu Sanhua’ | |
C21 | O. fragrans ‘Foding Zhu’ | |
C22 | O. fragrans ‘Tianxiang Taige’ |
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Wang, Y.; Fu, J.; Zhang, C.; Zhao, H. HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus. Molecules 2016, 21, 1224. https://doi.org/10.3390/molecules21091224
Wang Y, Fu J, Zhang C, Zhao H. HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus. Molecules. 2016; 21(9):1224. https://doi.org/10.3390/molecules21091224
Chicago/Turabian StyleWang, Yiguang, Jianxin Fu, Chao Zhang, and Hongbo Zhao. 2016. "HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus" Molecules 21, no. 9: 1224. https://doi.org/10.3390/molecules21091224