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Molecules 2016, 21(9), 1224; doi:10.3390/molecules21091224

HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus

Department of Ornamental Horticulture, School of Landscape Architecture, Zhejiang Agriculture and Forestry University, Lin’an 311300, China
Authors to whom correspondence should be addressed.
Academic Editor: Derek J. McPhee
Received: 11 August 2016 / Revised: 7 September 2016 / Accepted: 8 September 2016 / Published: 14 September 2016
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Osmanthus fragrans Lour. has traditionally been a popular ornamental plant in China. In this study, ethanol extracts of the leaves of four cultivar groups of O. fragrans were analyzed by high-performance liquid chromatography coupled with diode array detection (HPLC-DAD) and high-performance liquid chromatography with electrospray ionization and mass spectrometry (HPLC-ESI-MS). The results suggest that variation in flavonoids among O. fragrans cultivars is quantitative, rather than qualitative. Fifteen components were detected and separated, among which, the structures of 11 flavonoids and two coumarins were identified or tentatively identified. According to principal component analysis (PCA) and hierarchical cluster analysis (HCA) based on the abundance of these components (expressed as rutin equivalents), 22 selected cultivars were classified into four clusters. The seven cultivars from Cluster III (‘Xiaoye Sugui’, ‘Boye Jingui’, ‘Wuyi Dangui’, ‘Yingye Dangui’, ‘Danzhuang’, ‘Foding Zhu’, and ‘Tianxiang Taige’), which are enriched in rutin and total flavonoids, and ‘Sijigui’ from Cluster II which contained the highest amounts of kaempferol glycosides and apigenin 7-O-glucoside, could be selected as potential pharmaceutical resources. However, the chemotaxonomy in this paper does not correlate with the distribution of the existing cultivar groups, demonstrating that the distribution of flavonoids in O. fragrans leaves does not provide an effective means of classification for O. fragrans cultivars based on flower color. View Full-Text
Keywords: Osmanthus fragrans Lour.; leaves; HPLC-DAD-ESI-MS; flavonoids; cultivar groups; chemotaxonomy Osmanthus fragrans Lour.; leaves; HPLC-DAD-ESI-MS; flavonoids; cultivar groups; chemotaxonomy

This is an open access article distributed under the Creative Commons Attribution License which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0).

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Wang, Y.; Fu, J.; Zhang, C.; Zhao, H. HPLC-DAD-ESI-MS Analysis of Flavonoids from Leaves of Different Cultivars of Sweet Osmanthus. Molecules 2016, 21, 1224.

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